Differential gene expression in abdomens of the malaria vector mosquito, Anopheles gambiae, after sugar feeding, blood feeding and Plasmodium berghei infection

Dana, Ali N; Hillenmeyer, Maureen E; Lobo, Neil F; Kern, Marcia K; Romans, Patricia A; Collins, Frank H

doi:10.1186/1471-2164-7-119

Table 6 ASSEMBLED SEQUENCES WITH STATISTICALLY SIGNIFICANT DIGITAL NORTHERN VALUES

From: Differential gene expression in abdomens of the malaria vector mosquito, Anopheles gambiae, after sugar feeding, blood feeding and Plasmodium berghei infection

AS	Sequence similarity; Description	S	RB	IRB	S (norm)	RB (norm)	IRB (norm)	AC S-RB	AC S-IRB	AC RB-IRB	R
996	agCP14019; similar to Aedes aegypti vitellogenic cathepsin-B like protease (VCB).	0	0	5	0	0	29		0.015	0.03	*0.007*
24	no known predicted protein; shares 96% nucleotide identity with transcript ENSANG00000021567, a gene product belonging to the vitelline membrane protein family	0	1	12	0	9	72	0.240	0	*0.004*	0
139	ebiP415; Ribosomal protein L44	6	5	17	35	44	102	0.132	*0.005*	0.017	0.034
475	agCP12050; AgMuc1	2	3	12	12	26	72	0.138	*0.002*	0.024	*0.012*
145	agCP6338; protein of unknown function containing a HMW kininogen protein domain	1	2	8	6	18	48	0.172	*0.008*	0.046	0.034
313	CG14235; Cytochrome c oxidase	1	2	8	6	18	48	0.172	*0.008*	0.046	0.034
270	no known predicted protein; shares sequence identitiy with transcript 17000687051196 A.Gam.ad.cDNA.blood 1 of unknown function	2	3	10	12	26	60	0.138	*0.007*	0.042	0.042
230	agCP2518; Vitellogenin 1	1	7	19	6	61	114	*0.005*	0	0.024	0
98	agCP4445; hydrogen-transporting two-sector ATPase	0	3	4	0	26	23	0.038	0.030	0.119	0.035
521	agCP8317; Ribosomal protein L13	7	14	15	41	122	90	*0.005*	0.018	0.043	0.039
447	agCP2518; Vitellogenin 1	0	10	8	0	87	48	0	*0.002*	0.034	0
35	Glutathione S-transferase D1-6	0	4	3	0	35	17	0.015	0.060	0.081	0.024
537	agCP5224; Glycine hydroxymethyltransferase	0	5	2	0	44	11	*0.006*	0.123	0.034	0.009
273	agCP12503; ATP synthase B chain mitochondrial precursor (FO-ATP synthase subunit B)	1	6	3	6	52	17	*0.010*	0.123	0.032	0.038
516	agCP3409; AgPer1	8	10	3	46	87	17	0.046	0.045	*0.003*	0.029
8	agCP8133; Ribosomal protein L38e	14	6	3	81	52	17	0.076	*0.003*	0.032	0.025
208	agCP7801; ATP dependent RNA helicase	5	1	0	28	9	0	0.113	0.017	0.166	0.030
28	agCP11956; Serine protease G13	17	3	0	98	26	0	0.008	0	0.027	0
591	agCP1095; peptide with no known function, is part of the protein Family mucin 4 tracheobronchial mucin fragment, shares weak identity to Cryptosporidium parvum mucin-like glycoprotein 900	6	1	0	35	9	0	0.079	*0.009*	0.17	*0.013*
113	agCP10139; 65 aa peptide of unknown function containing no known protein domains except signal peptide and transmembrane regions.	8	0	0	46	0	0	*0.010*	*0.002*		0
18	agCP11264; Trypsin 1	25	2	4	145	18	23	0	0	0.125	0
408	17000687367332 A.Gam.ad.cDNA1; EST of unknown function	5	0	0	28	0	0	0.047	0.017		*0.008*
13	gene ENSANGG0000002077 6; gene located 4 kb 3' to the Peritrophin 1 that encodes a protein with a chitin binding domain	16	2	5	93	18	29	*0.004*	*0.006*	0.104	*0.007*
99	agCP3123; Chymotrypsin 2	16	3	8	93	26	48	*0.01*	0.025	0.069	0.054
170	agCP12309; putative homolog of Drosophila melanogaster Heat shock 70 kDa protein cognate 4 (Heat shock 70 kDa protein 88E)	7	0	4	41	0	23	0.017	0.086	0.050	0.028

Actual EST counts (S, RB and IRB) and the normalized number of ESTs (S (norm), RB (norm), and IRB (norm)) are given for each cDNA library. Assembled sequences were identified as differentially expressed among the three libraries if the R statistic was less than 0.05 (Stekel et al. 2000). The Audic and Claverie statistic (AC < 0.05) was used to identify libraries in which the assembled sequence was statistically significantly differentially expressed (Audic and Claverie, 1997). Pairwise comparisons between libraries are indicated by AC S-RB, AC S-IRB, and AC RB-IRB, respectively. All statistics were calculated with the IDEG6 Tool (Romualdi et al., 2003). Cells with characters in bold (underlined), bold (italics) and bold indicate statistical values equal to zero, less than 0.01, and less than 0.05, respectively. Cells with characters in italics (only), represent different levels of gene expression ranging from no detectable transcripts (smallest) to 145 transcripts (largest) detected in a library.

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ISSN: 1471-2164

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