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Table 6 ASSEMBLED SEQUENCES WITH STATISTICALLY SIGNIFICANT DIGITAL NORTHERN VALUES

From: Differential gene expression in abdomens of the malaria vector mosquito, Anopheles gambiae, after sugar feeding, blood feeding and Plasmodium berghei infection

AS Sequence similarity; Description S RB IRB S (norm) RB (norm) IRB (norm) AC S-RB AC S-IRB AC RB-IRB R
996 agCP14019; similar to Aedes aegypti vitellogenic cathepsin-B like protease (VCB). 0 0 5 0 0 29   0.015 0.03 0.007
24 no known predicted protein; shares 96% nucleotide identity with transcript ENSANG00000021567, a gene product belonging to the vitelline membrane protein family 0 1 12 0 9 72 0.240 0 0.004 0
139 ebiP415; Ribosomal protein L44 6 5 17 35 44 102 0.132 0.005 0.017 0.034
475 agCP12050; AgMuc1 2 3 12 12 26 72 0.138 0.002 0.024 0.012
145 agCP6338; protein of unknown function containing a HMW kininogen protein domain 1 2 8 6 18 48 0.172 0.008 0.046 0.034
313 CG14235; Cytochrome c oxidase 1 2 8 6 18 48 0.172 0.008 0.046 0.034
270 no known predicted protein; shares sequence identitiy with transcript 17000687051196 A.Gam.ad.cDNA.blood 1 of unknown function 2 3 10 12 26 60 0.138 0.007 0.042 0.042
230 agCP2518; Vitellogenin 1 1 7 19 6 61 114 0.005 0 0.024 0
98 agCP4445; hydrogen-transporting two-sector ATPase 0 3 4 0 26 23 0.038 0.030 0.119 0.035
521 agCP8317; Ribosomal protein L13 7 14 15 41 122 90 0.005 0.018 0.043 0.039
447 agCP2518; Vitellogenin 1 0 10 8 0 87 48 0 0.002 0.034 0
35 Glutathione S-transferase D1-6 0 4 3 0 35 17 0.015 0.060 0.081 0.024
537 agCP5224; Glycine hydroxymethyltransferase 0 5 2 0 44 11 0.006 0.123 0.034 0.009
273 agCP12503; ATP synthase B chain mitochondrial precursor (FO-ATP synthase subunit B) 1 6 3 6 52 17 0.010 0.123 0.032 0.038
516 agCP3409; AgPer1 8 10 3 46 87 17 0.046 0.045 0.003 0.029
8 agCP8133; Ribosomal protein L38e 14 6 3 81 52 17 0.076 0.003 0.032 0.025
208 agCP7801; ATP dependent RNA helicase 5 1 0 28 9 0 0.113 0.017 0.166 0.030
28 agCP11956; Serine protease G13 17 3 0 98 26 0 0.008 0 0.027 0
591 agCP1095; peptide with no known function, is part of the protein Family mucin 4 tracheobronchial mucin fragment, shares weak identity to Cryptosporidium parvum mucin-like glycoprotein 900 6 1 0 35 9 0 0.079 0.009 0.17 0.013
113 agCP10139; 65 aa peptide of unknown function containing no known protein domains except signal peptide and transmembrane regions. 8 0 0 46 0 0 0.010 0.002   0
18 agCP11264; Trypsin 1 25 2 4 145 18 23 0 0 0.125 0
408 17000687367332 A.Gam.ad.cDNA1; EST of unknown function 5 0 0 28 0 0 0.047 0.017   0.008
13 gene ENSANGG0000002077 6; gene located 4 kb 3' to the Peritrophin 1 that encodes a protein with a chitin binding domain 16 2 5 93 18 29 0.004 0.006 0.104 0.007
99 agCP3123; Chymotrypsin 2 16 3 8 93 26 48 0.01 0.025 0.069 0.054
170 agCP12309; putative homolog of Drosophila melanogaster Heat shock 70 kDa protein cognate 4 (Heat shock 70 kDa protein 88E) 7 0 4 41 0 23 0.017 0.086 0.050 0.028
  1. Actual EST counts (S, RB and IRB) and the normalized number of ESTs (S (norm), RB (norm), and IRB (norm)) are given for each cDNA library. Assembled sequences were identified as differentially expressed among the three libraries if the R statistic was less than 0.05 (Stekel et al. 2000). The Audic and Claverie statistic (AC < 0.05) was used to identify libraries in which the assembled sequence was statistically significantly differentially expressed (Audic and Claverie, 1997). Pairwise comparisons between libraries are indicated by AC S-RB, AC S-IRB, and AC RB-IRB, respectively. All statistics were calculated with the IDEG6 Tool (Romualdi et al., 2003). Cells with characters in bold (underlined), bold (italics) and bold indicate statistical values equal to zero, less than 0.01, and less than 0.05, respectively. Cells with characters in italics (only), represent different levels of gene expression ranging from no detectable transcripts (smallest) to 145 transcripts (largest) detected in a library.