High resolution physical map of porcine chromosome 7 QTL region and comparative mapping of this region among vertebrate genomes

Table 2 Bovine markers developed for RH mapping

STS-EST	SEQUENCE UP	SEQUENCE DN	SIZE OF PCR FRAGMENT	% RETENTION ON RH5000	POSITION (cR)	HUMAN LOCATION (Mb)
KHDRBS2	ATGGGGTTGCAAACAAGAAG	TTTGACAAATTGTGGTTTCCT	300	36	0	62.75
PRIM2A	AAAGCAGCATTCTGGACTGG	CCAGAAAAGGTCTGGGTCAA	104	40	82	57.29
BAG2	AATCTGACTGCGAACCGTCT	GTTTCCCAGGTCATCCAGAA	150	36	94	57.15
DST	CCACCTGGACCTGAGACCTA	ACAGGCGCTACTGTCCACTC	101	39	106	56.58
COL21A1	CAGTTCTTCGGAGCCTTGAG	GACTGCAACTGACTGGCTGA	133	34	125	56.12
TINAG	ACCGCATAGCAATTCAGTCC	ACCACCAAGCCCTATCAACA	123	31	184	54.28
LANO	CGGTCTCCAGCTTGATTGTT	TCCAAGAGAAGGTGGGTTTG	185	37	202	53.77
RAB2L	TCAATCTGGTCCCCTCTCAG	CCAGGACAGAGCTGACCACT	141	33	230	33.37
B3GALT4	GGAGGCAAGCACCAGGTAT	CTGGCCACCTTCAGGATAAG	125	37		33.35
RING1	ATCCAAAGTGACCCCACAAG	GGCAATAGAAAAGGCAGCAG	228	37		33.29
HKE4	GACTATGTCCGTGGTGCAAA	CTGTACGAAGACCTGCACGA	181	37		33.28
RXRB	CTGAAAAAGGTGGTGGTGGT	CATAAGAGCCGCAGAGAACC	193	37		33.27
VPS52	ACCCAGCTGATCCAGCTCTA	ATCAGAAGTTGGGCTTGTGC	138	29		33.33
ELOVL5	TCATGCTTTGATTTTGCACAT	TGTAAACACGAAGCCGTGACT	104	28		53.28
PTP4A1	GTGATTCCCAGCCTCTCTTG	CCACTTGTTCCCGGACAGTA	199	50		64.35
PHF3	CCATTTCGAAGAGGATCAGC	AACCCAGGTGGGCTAGACTT	188	37		64.45

Markers in bold belong to the framework RH map whereas markers in italic are positionned with the best likelihood. Primer sequences, retention fraction, RH map location on bovine map and human location are indicated.

ISSN: 1471-2164