A wing expressed sequence tag resource for Bicyclus anynana butterflies, an evo-devo model

BMC Genomics

Table 4 GO functional terms for B. anynana genes annotated to Drosophila CG numbers

GO TERMS	#	%	P-value
Molecular function
Signal transducer activity (GO: 0004871)	72	7	0.3025
Structural molecule activity (GO: 0005198)	195	25
Motor activity (GO: 0003774)	15	16
Catalytic activity (GO: 0003824)	568	14
Transporter activity (GO: 0005215)	151	12	0.3294
Binding (GO: 0005488)	651	15
Antioxidant activity (GO: 0016209)	13	33
Enzyme regulator activity (GO: 0030528)	52	14
Transcription regulator activity (GO: 0030528)	103	12	0.0011
Transcription factor activity (GO: 0003700)	28	7
Translation regulator activity (GO: 0045182)	26	28
Biological process
Development (GO: 0007275)	190	8
Larval or pupal development (GO: 0002165)	57	10
Pattern specification (GO: 0007389)	37	10
Metamorphosis (GO: 0007552)	43	9
Aging (GO: 0007568)	5	10
Pigmentation (GO: 0048066)	4	7
Regulation of development (GO: 0050793)	5	7
Morphogenesis (GO: 0009653)	89	9	0.1183
Embryonic development (GO: 0009790)	46	9
Physiological process (GO: 0007582)	1013	13
Behavior (GO: 0007610)	31	8
Cellular process (GO: 0009987)	1000	13
Regulation of biological process (GO: 0050789)	217	13
Highlights
Structural constituent of ribosome (GO: 0003735)	110	57
Tubulin (GO: 0045298)	4	25
Wing disc development (GO: 0035220)	21	11
Tracheal system development (GO: 0007424)	13	10
Wnt receptor signaling pathway (GO: 0016055)	11	15
sevenless signaling pathway (GO: 0045500)	2	22
Notch signaling pathway (GO: 0007219)	3	7
frizzled signaling pathway (GO: 0007222)	6	29
Eye pigment metabolism (GO: 0042441)	4	13

# is the number of B. anynana gene annotations obtained via BLAST analysis D. melanogaster and B. mori (total CG numbers in B. anynana with GO annotation = 1,164) that belong to the listed GO categories. % refers to the proportion of D. melanogaster genes (total CG numbers in D. melanogaster with GO annotation = 10,391) found in B. anynana for each GO category. The categories specified in this list are a limited subset of all GO categories found to be represented [see complete list in Additional file 7]. A Chi-square test was performed for the null hypothesis that the fraction of B. anynana UniGenes in each GO category relative to the total number of B. anynana UniGenes with an assigned category is equivalent to the fraction of D. melanogaster genes in that category relative to all D. melanogaster genes with a GO assignment. For the cases where Chi-square p > 0.0001, p-values are provided. All other cases are p < 0.0001.

ISSN: 1471-2164