Table 3 List of human target genes of (murine) Gfi-1 which contain Alu in their 5' upstream region. In these genes some of the Gfi-1 target sites are on Alu sequences; such sequences are denoted by '+' in the third column. The first column contains the target gene name; the second column is a potential binding sequence in the promoter region of the gene. The fourth column is a result of EMSA binding experiment for the sequence in column 2 [41].

AZU

1, 5'-CTCCCAAAGTGCTGAGATTA CAGGTGTGAG-3'^†

+

+

2, 5'-CGGCCTGGAGACCAGATTT GAGCCCTGGGA-3'

-

+

3, 5'-ACTTCCTGCCTCTGCAGATTT GCTTGTTCT-3'

-

-

4, 5'-TTTCACACCTGTAATCCCAG CATTTTGGGA-3'

+

+

5, 5'-GGTGGACAACTGTAATCCTAG CTACTCAGG-3"

-

-

AAT

1, 5'-GCACACGCCTGTAATCCCAG CTATTTGGGA-3'

+

+

2, 5'-CCCAGGAGGTTTAATCACAG CCCCTCCATG-3'*

-

+

3, 5'-ATAGCTGACCTAATCTCTC TGGCTTTGGTT-3'*

-

-

ACT

1, 5'-GCTCATGCCTGTAATCCCAG CACTTTGGGA-3'

+

+

2, 5'-TTCTTGGCTGCCACTGATTCCCTGTGCCTT-3'

-

+

3, 5'-GCCAAAATAAAATCCCAG TCCAAGTCTGGG-3'*

-

+

Jak3

1, 5'-CTCACGTGTGTAATCACAG CACTTTGGG-3'

+

+

P21

1, 5'-GCATGCCTGTAATCCCAG CTACTCGGG-3'

+

+

2, 5'-ATGTCTGGGCAGAGATTT CCAGACTCT-3'

-

+