Figure 1From: Quantify single nucleotide polymorphism (SNP) ratio in pooled DNA based on normalized fluorescence real-time PCREffect of fluorescence normalization. (a) Signal FAM curves of four replicates. (b) Same FAM data as shown in (a) after baseline (cycle 4~20) subtraction. (c) Amplification curve (FAM) of four replicates after fluorescence normalization.Back to article page