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Table 4 intra-run and inter-run variations of three methods for three different runs

From: Quantify single nucleotide polymorphism (SNP) ratio in pooled DNA based on normalized fluorescence real-time PCR

allele ratio Kf method Baseline subtracted fluorescence ratio method SDM method (2-ΔCt method)
  Run 1 Run 2 Run 3 Combined Run 1 Run 2 Run 3 Combined Run 1 Run 2 Run 3 Combined
  intra C.V. intra C.V. intra C.V. inter C.V. intra C.V. intra C.V. intra C.V. inter C.V. intra C.V. intra C.V. intra C.V. inter C.V.
90%:10% 4.38% 4.90% 11.80% 7.69% 9.39% 4.91% 11.95% 30.98% 13.81% 37.98% 47.29% 69.23%
80%:20% 9.18% 16.15% 5.45% 12.54% 5.30% 9.11% 6.45% 21.53% 26.72% 20.39% 3.30% 23.96%
70%:30% 5.40% 6.66% 17.22% 13.64% 18.48% 26.65% 31.87% 26.26% 11.90% 10.05% 9.32% 10.36%
60%:40% 2.99% 4.44% 6.11% 10.91% 4.41% 10.11% 12.22% 11.55% 3.52% 7.41% 24.43% 15.06%
50%:50% 6.06% 24.75% 7.30% 23.71% 10.59% 45.08% 15.59% 41.63% 22.12% 31.72% 10.38% 27.37%
40%:60% 28.91% 27.05% 7.77% 20.61% 33.55% 34.65% 13.09% 34.48% 38.09% 13.30% 8.56% 24.53%
30%:70% 4.70% 15.97% 1.91% 20.19% 7.38% 22.71% 4.89% 41.05% 7.61% 11.17% 25.12% 26.06%
20%:80% 29.02% 18.71% 3.67% 17.82% 36.42% 10.93% 6.57% 30.18% 13.72% 13.23% 42.29% 28.82%
10%:90% 61.69% 22.86% 10.24% 44.25% 81.13% 21.84% 5.98% 44.52% 98.39% 21.62% 113.30% 103.45%
  1. Further detailed Kf values were provided as supplementary material 2, 3 for run1 [see Additional file 2, 3], supplementary material 4, 5 for run 2 [see Additional file 4, 5], supplementary material 6,7 for run 3 [see Additional file 6, 7].