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Table 1 Hybridization signal strength of five genes found to be expressed using the array tube but not using the Affymetrix chip, respectively, compared with the results of quantitative PCR experiments (non-stimulated Coga cells). Array tube: relative transmission values, normalized overall. Affymetrix chip: relative fluorescence value, normalized overall

From: An alternative method to amplify RNA without loss of signal conservation for expression analysis with a proteinase DNA microarray in the ArrayTube® format

Gene

Affymetrix chip

Array tube

PCR

Quantitative RT-PCR

PAI-1

not present

0,53

 

4,34*10-7 ng

MMP7

not present

0,52

 

1,02*10-6 ng

MMP8

not present

0,47

positive

 

CST5

not present

0,89

positive

 

CTSK

273,8+)

0,43

 

8,13*10-6 ng

  1. +) this value is below the cut off of 300.