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Table 2 Relative expression level of selected genes (fluorescence and transmission values, respectively) versus expression level determined by quantitative RT-PCR (non stimulated cell line)

From: An alternative method to amplify RNA without loss of signal conservation for expression analysis with a proteinase DNA microarray in the ArrayTube® format

gene

Affymetrix chip

Array tube

Quantitative RT-PCR

ST14

1000; 3000

1,6

6*10-3 ng

MMP1

6320,6

1,2

3,6*10-4 ng

TIMP3

619; 976; 1564,5

1,15

2,2*10-4 ng

CTSK

273,8 +)

0,43

8,13*10-6 ng

PAI-1

Not present

0,84

4,34*10-7 ng

  1. +) this value is below the cut off of 300.