Figure 1

Overview of the design and use of the large-scale cell-based microarray for over-expression studies. a. Representative agarose gel image of plasmids prepared from 2,976 MGC (IRAT) clones. b. Array was designed such that each clone was printed in quadruplicate (yellow and red squares) surrounded by columns of GFP vector (green squares). The position of GFP-tagged positive control genes is shown by small white boxes. c. 1,959 plasmids in 0.3% gelatin were printed on to a glass slide in to form an array with 9,888 features. The image is of an array scanned directly after printing (Agilent microarray scanner). d. An array cultured with HEK293T cells and scanned with a fluorescent imager (GE Healthcare, Typhoon) to show lines of GFP positive cells. e. Arrays were subjected to a functional assay to detect changes in the cell after over-expression of proteins. The image is of TUNEL positive cells, scale bar = 10 μm.