Table 1 Primer information (sequence, melting temperature and PCR product length) F:Forward primer, R: Reverse primer
|  | GenBank Nr. | Sequence | Tm [°C] | PCR products: normal/alternative isoform [bp] |
|---|---|---|---|---|
CTNNB1(a, b) | NM_001904 | F:GACCTCATGGATGGGCTG R:AGGCTAGGGTTTGCTAAATTCC | 60.0 60.0 | 329/168 |
CtBP1 | NM_001328 | F:GATGGGCAGCTCGCACTT R: TTCATGATCGGAGGTCGGAC | 59.0 59.0 | 63/257 |
GSK-3β | NM_002093 | F:AATGAACCCAAACTACACAGAATTTAAA R:CAATTGCCTCCGGTGGAGT | 59.0 59.0 | 135/96 |
Axin-1 | NM_003502 | F-A:GCGCCGGTGCTGCA R-A: ACCCCACAGTCAAACTCGTC F-B:GGAGGAGGAAGAAAAGAGAGCC R-B:TGCCGATGATCTTCTCCTCAA | 60.0 62.0 68.0 62.0 | A:264, only normal isoform B:428/286 |
Axin-1, real-time PCR | NM_003502 | F-T1:AGCCGTGTCGGACATGGA R-T1:AAGTAGTACGCCACAACGATGCT F-T2:GCACCCTCCAAGCAGAGAGA R-T2:CGCAGAAGTAGTACGCCACAAC | 59.6 58.9 59.1 58.6 | T1:104 T2:94 |
LRP 5 | NM_002335 | V-A: CCCTCTACTCACCCATGGACA R-A: AGGCACAGGTGGGAGCAG V-B: CAGCACCCGGAAGATCATTGT R-B: TCGTTGATCTCGGTGTTGACC | 58 58 61 60 | A:103, only normal isoform B:702/571 |