Verification of predicted alternatively spliced Wnt genes reveals two new splice variants (CTNNB1 and LRP5) and altered Axin-1 expression during tumour progression

Table 2 PCR conditions, thermal cycling programs

	CTNNB1		Ctbp1 (1)		Ctbp1 (2)		LRP 5
	°C	min	°C	min	°C	min	°C	min
activation	94	3	94	3	94	3	94	3
Steps:
strand separation	90	1,5	90	1,5	92	2	92	1,5
annealing	66	1	58	1	59	1	64	1
elongation	72	1	72	1,5	72	1,5	72	1,5
number of cycles	33		30		30		30
finale elongation	72	10	72	10	72	10	72	10
	GSK 3β		Axin-1 (A)		Axin-1 (B)
	°C	min	°C	min	°C	min
activation	94	3	94	3	94	3
Steps:
strand separation	90	1,5	92	1,5	90	2
annealing	63	1	63	1	65	1
elongation	72	1	72	1,5	72	1,5
number of cycles	30		30		30
finale elongation	72	10	72	10	72	10

ISSN: 1471-2164