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Table 1 Summary of tissues used for the different cDNA libraries generated for the FGAS EST sequencing project.

From: Wheat EST resources for functional genomics of abiotic stress

Library

Growth conditions*

Tissues

High quality EST sequences

Library 2

Control plants;

Plants cold acclimated for 1, 23 and 53 days

leaves and crowns

25,240

Library 3

Control plants;

Plants cold acclimated for 1, 23 and 53 days;

Plants salt stressed for 0.5, 3 and 6 hours

roots

11,382

Library 4

Plants dehydrated on the bench (4 time points) and in a growth chamber (4 time points)

leaves and crowns

2,838

Library 5

Various vernalization and developmental stages through spike formation.

crowns and flowers

6,668

Library 6

Control plants;

Plants cold acclimated for short time points (1, 3 and 6 hours) under light or dark conditions

leaves and crowns

7,904

TaLT2

SSH library: Tester: cv. CI14106 cold acclimated for 1 day; Driver: cv Norstar cold acclimated for 21 and 49 days

crowns

2,271

TaLT3

SSH library: Tester: cv. CI14106 cold acclimated for 21 and 49 days; Driver: cv Norstar cold acclimated for 1 day

crowns

1,832

TaLT4

SSH library: Tester: cv. PI178383 cold acclimated for 1 day; Driver: cv Norstar cold acclimated for 21 and 49 days

crowns

2,716

TaLT5

SSH library: Tester: cv. PI178383 cold acclimated for 21 and 49 days; Driver: cv Norstar cold acclimated for 1 day

crowns

2,784

TaLT6

SSH library: Tester: cv. CI14106 cold acclimated for 1 day; Driver: non-acclimated cv. CI14106

crowns

4,961

TaLT7

SSH library: Tester: cv. CI14106 cold acclimated for 21 and 49 days; Driver: non-acclimated cv. CI14106

crowns

4,925

  1. * Libraries 2 to 6 were constructed from wheat cv Norstar.