Figure 3

Novel gene discovery. Large increases in sequence contributions are evident from each set of libraries, which are illustrated as follows: sequences from 5 non-normalized libraries (approximately the first 10,000 ESTs); sequences from 5 normalized libraries (approximately from 10,000 to 30,000 ESTs); and sequences from pooled, subtracted libraries (after 30,000 ESTs) (Table 1). Novelty rates, calculated as the ratio of the total number of clusters identified over the total number of ESTs generated, were 39%, 54%, and 75%, for the non-normalized, normalized and subtracted libraries, after production of a total of 10,000 (3,920 clusters), 20,190 (10,822 clusters), and 5,171 (3,917 clusters) ESTs, respectively.