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Table 2 Reverse transcriptase-PCR analysis of the expression of clusters and contigs differentially expressed between the L1 and iL3s stages.

From: A microarray analysis of gene expression in the free-living stages of the parasitic nematode Strongyloides ratti

Cluster1 Fold1 Contig1 Fold1 RT-PCR mean (SD)2
SR00369 3.6 SR01101 3.89 0.84 (0)
SR00073 2.46 SR01013 2.46 1.02 (0)
SR00647 3.68 SR00647 3.68 3.13 (3.7)
SR00369 3.6 SR01099 3.1 4.34 (4.2)
SR00001 2.83 SR00001 5.82 4.18 (0.2)
SR00369 3.6 SR01100 5.82 1.7 (1.0)
SR00762 3.2 SR00762 3.2 18.7 (0.14)
SR00369 3.6 SR00853 4.24 5.74 (6.6)
SR00222 4.46 SR00222 4.45 18.3 (5.8)
SR03050 3.51 SR03050 3.51 2.69 (2.3)
SR01720 2.93 SR01720 2.93 9.7 (3.9)
SR00448 2.60 SR00448 2.59 9.6 (12.5)
SR01335 2.22 SR01335 2.22 3.63 (4.0)
SR00906 2.06 SR00906 2.06 3.39 (2.8)
SR02481 1.85 SR02481 1.85 7.61 (0.71)
SR01791 1.64 SR01791 1.64 2.4 (1.3)
SR00681 1.52 SR00681 1.52 4.3 (3.7)
SR01787 1.42 SR01787 1.42 2.72 (3.7)
SR00322 1.16 SR00322 1.16 8.08 (9.6)
SR00026 2.06 SR00172 2.88 6.71 (0)
SR00026 2.06 SR00047 2.33 1.77 (0.22)
SR00012 2.27 SR01022 2.74 0.12 (0)
SR01012 2.5 SR01012 2.5 6.5 (0)
SR00946 1.96 SR01050 2.02 8.75 (10.3)
SR00012 2.27 SR00685 3.1 15.9 (20.1)
SR01053 1.32 SR01053 1.32 2.44 (2.9)
SR00880 3.13 SR00880 3.13 5.63 (6.9)
SR00978 2.2 SR00978 2.2 2.86 (2.8)
SR01196 3.65 SR01196 3.66 10 (8.5)
SR02281 2 SR02281 2 3.22 (3.2)
SR00930 1.87 SR00930 1.87 1.04 (0.31)
SR01051 1.71 SR01051 1.71 1.18 (0.9)
SR00957 1.44 SR00957 1.44 3.41 (1.9)
SR02851 1.43 SR02851 1.43 1.45 (0.32)
SR00194 1.42 SR00194 1.42 0.28 (0.39)
SR00084 1.39 SR01021 1.39 6.34 (5.5)
SR00795 1.2 SR00795 1.2 3.97 (0)
SR00230 1.11 SR00230 1.11 5.39 (2.7)
SR00163* 1.91 SR00785 3.12 0.89 (0.12)
SR00113* 1.54 SR00113 - 0.99 (0.15)
  1. 1Mean cluster and contig fold difference in expression between L1 and iL3 stages measured by microarrays for iL3-up (normal text) and L1-up (bold text) clusters and contigs and for reference genes (*); – indicates that there were no significant data generated for this contig.
  2. 2The mean (SD) fold difference in expression as measured by RT-PCR is calculated as iL3s/L1 for iL3-up (normal text) contigs and clusters and L1/iL3 for L1-up (bold text) clusters and contigs and for the reference genes (*).