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Table 7 Reverse transcriptase-PCR analysis of the expression of clusters and contigs differentially expressed between the directly and indirectly developing L2s.

From: A microarray analysis of gene expression in the free-living stages of the parasitic nematode Strongyloides ratti

Cluster1

Fold1

Contig1

Fold1

RT-PCR Mean (SD)2

SR02363

2.08

SR02363

2.08

1.45 (0.46)

SR00009

1.69

SR00027

1.69

0.76 (0.45)

SR00113

1.60

SR00113

1.58

1.30 (0.67)

SR00113

1.60

SR00987

1.63

1.21 (0.18)

SR00013

1.25

SR00053

1.26

0.95 (0.48)

SR02856

1.48

SR02856

1.48

2.69 (2.06)

SR00395

1.46

SR00395

1.46

1.81 (2)

SR00012

1.40

SR00989

1.45

0.66 (0.17)

SR00012

1.40

SR00665

1.39

0.99 (0.59)

SR01030

1.39

SR01030

1.39

1.09 (0.6)

SR00008

1.31

SR00752

1.31

1.81 (1.01)

SR01870

1.34

SR01870

1.34

1.59 (2.06)

SR00591

1.30

SR00591

1.30

0.75 (0.33)

SR00012

1.40

SR00955

1.28

1.30 (0.35)

SR00055

1.23

SR00962

1.23

0.97 (0.14)

SR00112

1.21

SR00112

1.21

3.86 (5.72)

SR00950

1.18

SR00950

1.18

1.46 (1.45)

SR01054

1.18

SR01054

1.18

2.98 (3.91)

SR00611

1.16

SR00611

1.16

4.38 (2.89)

SR00038

1.22

SR00038

1.13

0.97 (0.51)

SR00205

1.01

SR00205

1.01

0.38 (0.33)

SR00170

2.58

SR00170

2.58

6.18 (1.76)

SR00374

2.02

SR00374

2.02

7.27 (4.57)

SR00351

1.73

SR00931

1.83

12.9 (20.3)

SR00713

1.74

SR00713

1.74

1.13 (0.36)

SR00775

1.68

SR00775

1.68

6.28 (8.64)

SR00199

1.45

SR00199

1.45

1.71 (0.22)

SR01250

1.38

SR01250

1.38

3.51 (0)

SR00799

1.35

SR00799

1.35

4.38 (4.28)

SR00811

1.28

SR00811

1.28

0.91 (0.3)

SR00092

1.21

SR00092

1.21

0.99 (0.24)

SR02792

1.21

SR02792

1.21

6.56 (7.21)

SR00721

1.19

SR00721

1.19

1.06 (0.27)

SR02972

1.17

SR02972

1.17

14.8 (23.1)

SR00994

1.16

SR00994

1.16

1.16 (0.43)

SR01156

1.57

SR01156

1.57

2.04 (2.06)

SR02019

1.84

SR02019

1.84

2.85 (3.94)

SR00178

1.39

SR00178

1.39

1.09 (0.11)

SR00342

1.14

SR00543

1.14

10.8 (1.5)

SR02917

1.1

SR02917

1.1

26.5 (41.6)

SR00615

1.15

SR00615

1.1

1.76 (0.65)

SR02394

1.07

SR02394

1.07

8.72 (1.75)

SR00817

1.06

SR00817

1.06

13.4 (1.11)

SR00266

1.06

SR00266

1.06

2.55 (2.5)

SR00451

1.02

SR00451

1.02

11.1 (16.2)

SR00113*

1.6

SR00113

1.58

1.23 (0.87)

SR00015*

1.1

SR00015

1.1

0.25 (0.01)

  1. 1Mean cluster and contig fold difference in expression between indirectly and directly developing L2 stages measured by microarrays for L2 direct-up (normal text) and L2 indirect-up (bold text) clusters and contigs and for reference genes (*).
  2. 2 The mean (SD) fold difference in expression as measured by RT-PCR is calculated as direct/indirect for the L2 direct-up (normal text) contigs and clusters and for the reference genes (*) and indirect/direct for the L2 indirect-up (bold text) contigs and clusters.
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BMC Genomics

ISSN: 1471-2164

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