Figure 3

Summary of tissue specific TRP channel gene expression. Expression of TRPC1-7, TRPM1-8, TRPV1-6 and TRPA1 mRNAs in murine tissues was determined by real-time RT-PCR. Levels of mRNAs are given in relation to that of 18S rRNA; all data were multiplied by 1000. Means were calculated from n = 3–10 samples and classified into 5 categories. If the threshold level of fluorescence according to the TaqMan RT-PCR technique was not reached after 40 cycles of amplification, a resulting expression level of zero (0) was assigned to the corresponding channel transcript and tissue. For better illustration categories of gene expression levels are represented by different colors ranging from zero (white) to values higher than 10 (dark blue).