Illustration of TDL_miRBase dataset report interface. (a) The complete information report for a candidate hairpin includes host gene, host gene NM accession number (for intronic candidates), genomic location, expression level and match to known miRNAs. The score and minimum free energy (mfe) are the output results from Srnaloop and RNAfold, respectively. (b) Target gene information for a candidate from hairpin Ih788. Target genes were discovered by RNAhybrid and pre-defined conserved motif seeds as described in the text. Optimal free energy and RNA duplex mfe are the output values of RNAhybrid. The GO information of the Ih788 host gene and one of its target genes are displayed in (c). (d) Orthologous target genes report. Some of the target genes were found to be orthologous pairs according to Ensembl gene information. They are displayed as human-mouse or human-rat pairs.