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Table 3 Supplemental genes investigated by RT-PCR for differential xpression. See Methods for abbreviations and Table 2 for explanations.

From: Gene identification and analysis of transcripts differentially regulated in fracture healing by EST sequencing in the domestic sheep

Gene

UniGene

EST Ratio

RT-PCR

Bone/Development

COL1A2

Hs.489142

522:210 (0.51; p = 1.2 × 10-12)

*/**/**

+/+ [60]

ITGB1

Hs.429052

8:21 (0.078; p= 1.5 × 10-8)

-/**/**

+/+ [78]

COL6A2

Hs.420269

111:56 (0.41; p = 2.8 × 10-5)

-/**/**

+/+ [79]

TAGLN2

Hs.517168

110:8 (2.8; p = 0.054)

-/**/**

-/-

CTSS

Hs.181301

34:0 (-; p = 0.069)

-/**/**

-/+ [80]

MORF4L1

Hs.374503

34:0 (-; p = 0.069)

-/*/**

-/-

GPX3

Hs.386793

36:0 (-; p = 0.073)

-/**/**

+/+ [17]

Endoglin

Hs.76753

32:0 (-; p = 0.087)

-/*/**

-/+ [30]

RPL9

Hs.412370

32:0 (-; p = 0.087)

-/**/**

-/+ [81]

CSRP1

Hs.108080

95:7 (2.8; p = 0.089)

-/**/**

-/+ [29]

RPS4X

Hs.446628

31:0 (-; p = 0.11)

-/*/**

-/-

RPL10a

Hs.546269

30:0 (-; p = 0.11)

-/**/**

-/-

RPL6

Hs.546283

30:0 (-; p = 0.11)

-/**/**

-/-

PPIB

Hs.434937

31:0 (-;p = 0.11)

-/**/**

+/+ [60]

ANXA2

Hs.511605

158:17 (1.9; p = 0.14)

*/**/**

+/+ [82]

JUNB

Hs.25292

27:0 (-;p = 0.15)

-/**/**

+/+ [83]

RPL17

Hs.374588

27:0 (-;p = 0.15)

-/**/**

-/-

TPM2

Hs.300772

28:0 (-;p = 0.16)

-/**/**

-/-

Lumican

Hs.406475

114:12 (1.9; p = 0.21)

*/**/**

+/+ [84]

MMP2

Hs.513617

53:20 (0.54; p = 0.21)

*/**/**

+/+ [85]

Perlecan

Hs.555874

45:2 (4.6; p = 0.21)

*/**/**

+/+ [86]

RPL41

Hs.381172

0:2 (-;p = 0.21)

*/**/**

-/-

GPX1

Hs.76686

20:0 (-;p = 0.26)

*/**/**

-/-

MMP13

Hs.2936

7:5 (0.29; p = 0.27)

**/**/**

+/+ [87]

GAPDH

Hs.544577

137:17 (1.7; p = 0.36)

-/*/**

-/-

RARA

Hs.535499

9:0 (-;p = 0.37)

-/*/*

+/+ [88]

ITGA5

Hs.505654

16:0 (-;p = 0.51)

*/**/**

+/+ [89]

BMP1

Hs.1274

16:0 (-;p = 0.51)

-/**/**

+/+ [90]

GPX7

Hs.43728

16:0 (-;p = 0.51)

-/**/**

-/-

LRP1

Hs. 162757

16:7 (0.47; p = 0.53)

-/**/**

-/-

COL4A2

Hs.508716

73:8 (1.9; p = 0.56)

*/**/**

-/-

GPX4

Hs.433951

13:0 (-;p = 0.59)

-/**/**

-/-

IBSP

Hs.518726

8:4 (0.41; p = 0.59)

**/**/**

+/+ [11]

RPL29

Hs.425125

0:1 (-;p = 0.59)

*/**/**

-/-

S100A4

Hs.81256

13:0 (-;p = 0.59)

-/**/**

+/+ [91]

ID1

Hs.504609

7:0 (-;p = 0.61)

-/*/*

+/+ [92]

KDELR2

Hs.520210

10:0 (-;p = 0.76)

-/**/**

-/-

MMP9

Hs.297413

11:0 (-;p = 0.76)

**/*/**

+/+ [35]

LRP10

Hs.525232

9:4(0.46; p = 0.83)

-/*/*

-/-

HDGF

Hs.506748

14:5 (0.57; p = 1.0)

-/*/*

-/+ [32]

COL6A1

Hs.474053

159:29 (1.1; p = 1.0)

-/-/*

+/+ [79]

CTSD

Hs.546248

87:13 (1.4; p = 1.0)

**/**/**

-/-

CTSK

Hs.523594

27:4 (1.4; p = 1.0)

*/**/**

+/+ [93]

ID3

Hs.76884

13:2 (1.3; p = 1.0)

-/*/*

+/+ [92]

KDELR3

Hs.554798

l:0 (-; p = 1.0)

-/**/**

-/-

MMP19

Hs. 154057

16:3 (1.1; p = 1.0)

-/**/**

-/-

TCF4

Hs.200285

5:0 (-;p = 1.0)

*/*/**

+/+ [47]

CST3

Hs.304682

12:2 (1.2; p = 1.0)

-/**/**

+/+ [37]