Figure 3
From: Expression of target and reference genes in Daphnia magna exposed to ibuprofen
Relative gene expression of target gene Ltb4dh in Daphnia magna following a 24 h exposure to ibuprofen. Mean relative expression (n = 3) ± SD of Ltb4dh following normalisation to 10 candidate reference genes and a normalisation factor (NF). Expression levels are displayed relative to the mean control level. For comparison, the normalised relative expression of Ltb4dh is displayed by treatment group. Relative expression of Ltb4dh normalised to: Act (1); Atb (2); Cyp (3); GAPDH (4); SDH (5); TBP (6); WARS (7); UBC (8); 18S (9); 28S (10); NF3 based on the geometric mean of Act, GAPDH and UBC (11); and Ltb4dh without normalisation (12) (gene name abbreviations as Table 1). An asterisk (*) indicates a significant difference (p < 0.05) from Ltb4dh normalised by NF3.