Figure 2
From: Comparative EST analysis provides insights into the basal aquatic fungus Blastocladiella emersonii
Northern blot analysis of B. emersonii genes encoding enzymes involved in propionyl-CoA metabolism. A, E. Propionyl-CoA carboxylase; B, F. DL-methylmalonyl-CoA-racemase; C, G. Methylmalonyl-CoA mutase; D, H. ATP:Cob(I)alamin adenosyltransferase. The RNA blots were also hybridized with a probe of the hsp70-3 gene, which is not induced by CoCl, as a control (A1-D1). Data from densitometry scanning of the hybridization bands is shown in panels E-H. The values were normalized using the hsp70-3 bands as a control. S = total RNA isolated from cells after 60 min of sporulation; S + Co = total RNA isolated from cells after 60 min of sporulation in the presence of 100 μM CoCl.