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Table 4 Validation of fold-changes from microarray analyses by real-time RT-PCR

From: Epigenetics of gene expression in human hepatoma cells: expression profiling the response to inhibition of DNA methylation and histone deacetylation

  

5-aza-dC

TSA

5-aza-dC and TSA

 

Control

Array1

RT-PCR1

Array

RT-PCR

Array

RT-PCR

CDK10 3

300*

3.5 (.00013)

8.3 (.00000)

NT2

NT

3.8 (.00001)

7.2 (.00000)

CEBPA 3

3106

NT

NT

1.43 (.0038)

4.16 (.004)

1.7 (.00049)

10.4 (.00000)

CEBPB 3

28789

-2.0 (.019)

-2.6 (.0008)

NT

NT

-2.0 (.019)

-3.5 (.00009)

CYP3A7 3

515*

4.0 (.0043)

11 (.00000)

NT

NT

4.3 (.0043)

8.0 (.00000)

GSTP1 3

367**

8.5 (.00014)

18200 (.00000)

NT

NT

12 (.00005)

107000 (.00000)

H19 3

255

156 (.00000)

5080 (.00000)

NT

NT

141 (.00000)

330 (.00000)

NNAT

205*

8.0 (.0073)

38 (.00000)

NT

NT

10.8 (.0053)

33 (.00000)

PKIB

464*

7.4 (.0029)

70 (.00000)

NT

NT

6.6 (.0013)

35 (.00000)

S100A4 3

281*

8.8 (.0012)

27 (.00000)

3.0 (.00039)

4.9 (.00000)

8.2 (.00018)

11 (.00000)

TSPY1

11*

96 (.00002)

532 (.00000)

NT

NT

153 (.00000)

1290 (.00000)

  1. *: Called "Absent" on all 4 control arrays; **: called "Absent" on 3 of 4 control arrays
  2. 1The fold-change for either the microarray analyses or real-time RT-PCR assays (relative fold-change). P-values (Welch's t-test) are shown in parentheses.
  3. 2NT = not tested, because it was not significantly affected in the microarray analyses.
  4. 3Expression is observed in the fetal liver.
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BMC Genomics

ISSN: 1471-2164

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