Figure 1

A. FAFLP analysis of H. pylori strains analyzed from native Peruvians (n = 27). The phylogenetic tree was developed based on various amplitypes generated for individual isolates after allele scoring and generation of similarity profiles in the form of binary tables. Genetic relationships in the form of a tree were deduced using MEGA 3.0 software using bootstrapping method at 10000 bootstrap trials. Two different lineages observed in the tree are colored as per the previous conventions [11, 20]. Six isolates from among those represented in the FAFLP tree (highlighted with circles) were also analyzed subsequently by MLST. B. Phylogenetic analysis of representative hsp-Amerind sequences from our isolates (SJM 83, 92) and those of other Amerind and non-Amerind (western) H. pylori sequences previously described by Ghose et al. from Caracas and Puerto Ayacucho in Venezuela [16]. SJM 83 and 92 formed a separate branch (maroon color) only with Amerind isolates (right). Both the Amerindian and western lineages (green color) are differentially colored as per previous conventions [11, 20]. Sequence alignment (left) of these Amerind sequences revealed significant conservation at the level of nucleotides.