Table 3 Gene ontology (GO) functional assignments for the libraries L02, L03, L06 and L10.

Biological Process

GO:0008150

809 (100%)

389 (100%)

211 (100%)

472 (100%)

Cellular process

GO:0009987

738 (91.2%)

352 (90.5%)

196 (92.9%)

434 (91.9%)

Regulation of biological process

GO:0050789

57 (7.0%)

23 (5.9%)

12 (5.7%)

42 (8.9%)

Response to stimulus

GO:0050896

39 (4.8%)

29 (7.5%)

7 (3.3%)

31 (6.6%)

Physiological process

GO:0007582

778 (96.2%)

370 (95.1%)

211 (100%)

452 (95.8%)

   Regulation of physiological process

GO:0050791

52 (6.4%)

20 (5.1%)

10 (4.7%)

36 (7.6%)

   Metabolism

GO:0008152

647 (80.0%)

303 (77.9%)

196 (92.9%)

364 (77.1%)

Biosynthesis

GO:0009058

275 (34.0%)

139 (35.7%)

119 (56.4%)

153 (32.4%)

Catabolism

GO:0009056

73 (9.0%)

33 (8.5%)

6 (2.8%)

29 (6.1%)

Cellular metabolism

GO:0044237

580 (71.7%)

267 (68.6%)

181 (85.8%)

324 (68.5%)

Macromolecule metabolism

GO:0043170

392 (48.4%)

187 (48.1%)

136 (64.4%)

228 (48.3%)

Nitrogen compound metabolism

GO:0006807

90 (11.1%)

32 (8.2%)

17 (8.1%)

40 (8.5%)

Primary metabolism

GO:0044238

533 (65.9%)

249 (64.0%)

170 (80.6%)

301 (63.6%)

Regulation of metabolism

GO:0019222

42 (5.2%)

15 (3.9%)

7 (3.3%)

28 (5.9%)

Secondary metabolism

GO:0019748

11 (2.8%)

3 (0.8%)

0

2 (0.4%)

Molecular Function

GO:0003674

837 (100%)

389 (100%)

228 (100%)

483 (100%)

Binding activity

GO:0005488

400 (47.8%)

165 (42.4%)

107 (46.9%)

223 (46.2%)

   Cofactor binding

GO:0048037

33 (3.9%)

15 (3.9%)

5 (2.2%)

16 (3.3%)

   Ion binding

GO:0043167

78 (9.3%)

34 (8.7%)

12 (5.3%)

46 (9.5%)

   Nucleic acid binding

GO:0003676

150 (17.9%)

65 (16.7%)

52 (22.8%)

82 (17.0%)

   Nucleotide binding

GO:0000166

133 (15.9%)

57 (14.7%)

32 (14.0%)

61 (12.6%)

   Protein binding

GO:0005515

54 (6.5%)

16 (4.1%)

18 (7.9%)

26 (5.4%)

Catalytic activity

GO:0003824

491 (58.7%)

232 (59.6%)

106 (46.5%)

284 (58.8%)

   Hydrolase activity

GO:0016787

162 (19.4%)

81 (20.8%)

33 (14.5%)

119 (24.6%)

   Lyase activity

GO:0016829

50 (6.0%)

23 (5.9%)

4 (1.8%)

15 (3.1%)

   Ligase activity

GO:0016874

38 (4.5%)

18 (4.6%)

9 (3.9%)

17 (3.5%)

   Oxidorreductase activity

GO:0016491

158 (18.9%)

71 (18.3%)

30 (13.2%)

78 (16.1%)

   Transferase activity

GO:0016740

124 (14.8%)

50 (12.9%)

31 (13.6%)

56 (11.6%)

Transporter activity

GO:0005215

121 (14.5%)

57 (14.7%)

18 (7.9%)

80 (16.6%)

   Carrier activity

GO:0005386

70 (8.4%)

32 (8.2%)

13 (5.7%)

49 (10.1%)

   Ion transporter activity

GO:0015075

50 (6.0%)

22 (5.7%)

12 (5.3%)

36 (7.5%)

Signal transducer activity

GO:0004871

16 (1.9%)

7 (1.8%)

5 (2.2%)

13 (2.7%)

Structural molecule activity

GO:0005198

80 (9.6%)

47 (12.1%)

63 (27.6%)

41 (8.5%)

Enzyme regulator activity

GO:0030234

18 (2.2%)

13 (3,3%)

5 (2.2%)

12 (2.5%)

Transcription regulator activity

GO:0030528

17 (2.0%)

8 (2.1%)

5 (2.2%)

12 (2.5%)

Translation regulator activity

GO:0045182

52 (6.2%)

22 (5.7%)

21 (9.2%)

42 (8.7%)

Cellular component

GO:0005575

563 (100%)

280 (100%)

156 (100%)

316 (100%)

Extracellular region

GO:0005576

26 (4.6%)

18 (6.4%)

1 (0.6%)

21 (6.6%)

Cell

GO:0005623

539 (95.7%)

266 (95.0%)

151 (96.8%)

300 (94.9%)

   Intracellular

GO:0005622

441 (78.3%)

211 (75.4%)

145 (92.9%)

235 (74.4%)

   Membrane

GO:0016020

180 (32.0%)

97 (34.6%)

24 (15.4%)

112 (35.4%)