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Table 2 Top 20 most redundant EST clusters.

From: Generation of a large scale repertoire of Expressed Sequence Tags (ESTs) from normalised rainbow trout cDNA libraries

Cluster Name

Cluster depth

Best swissprot hit

Hit description

Over-expressed in Agenae libraries

BX072800.1.p.om.3

1463

P48831

Zona pellucida sperm-binding protein 3 precursor

Yes (Ovary)

CR361581.1.p.om.3

1460

NULL

 

Yes (Ovary)

CA352033.1.p.om.3

1434

P10995

Actin, alpha sarcomeric/cardiac (Actin alpha 2)

No

BX073087.1.p.om.3

431

P21993

Prolactin precursor (PRL)

No

CA342041.1.p.om.3

402

P02141

Hemoglobin beta-4 subunit

No

CA369471.1.p.om.3

389

P30042

ES1 protein homolog, mitochondrial precursor (Protein KNP-I)

Yes (Pooled-Tissue)

CA368365.1.p.om.3

342

P35031

Trypsin I precursor

Yes (Pooled-Tissue)

BX074651.1.p.om.3

314

P20332

Somatotropin 2 precursor (Growth hormone 2)

No

CA341574.1.p.om.3

264

P35979

60S ribosomal protein L12

No

CA343108.1.p.om.3

255

P02609

Myosin regulatory light chain 2, skeletal muscle isoform (G2)

No

BX073970.1.p.om.3

224

P07064

Somatotropin precursor (Growth hormone)

No

CA341678.1.p.om.3

197

P70083

Sarcoplasmic/endoplasmic reticulum calcium ATPase 1

No

CA341906.1.p.om.3

178

Q90YV7

60S ribosomal protein L11

No

CA341950.1.p.om.3

173

Q90Z10

60S ribosomal protein L13

No

CA341578.1.p.om.3

170

P0AFB7

Nitrogen regulation protein NR(II)

No

CA345253.1.p.om.3

161

P47954

Glutathione S-transferase P

No

CA341772.1.p.om.3

159

O57561

60S ribosomal protein L18a

No

CA342754.1.p.om.3

157

Q91487

60S ribosomal protein L13a (Transplantation antigen P198 homolog)

No

CA342547.1.p.om.3

155

P02457

Collagen alpha 1(I) chain precursor

No

CA378499.1.p.om.3

153

NULL

 

No

  1. The 20 most redundant EST clusters in all rainbow trout cDNA libraries are listed with their Sigenae cluster name and the number of ESTs within each cluster (cluster depth). When a homology search using blastx was carried out against the Swissprot database returned a significant homology (blast score > 100), the accession number of this best putative homolog is given along with its associated description. When a cluster contains an over-representation of ESTs found only in one Agenae library, the name of this library is given in the last column.