Table 2 SYBR Green (a) and Taq Man primers (b) sequences used for RT PCR reactions.
From: Comprehensive analysis of the renal transcriptional response to acute uranyl nitrate exposure
GeneSymbol | Accession No. | Primer 5' -> 3' sequence | Amplicon size (bp) |
|---|---|---|---|
a. Primers using SYBR Green detection | |||
Hprt | NM_013556 | Forward 5'-TTGCTGACCTGCTGGATTAC-3' | 112 |
| Â | Â | Reverse 5'-CCCGTTGACTGATCATTACA-3' | Â |
Sod1 | XM_128337 | Forward 5'-TGGTGGTCCATGAGAAACAA-3' | 75 |
| Â | Â | Reverse 5'-TCCCAGCATTTCCAGTCTTT-3' | Â |
Ftl1 | NM_010240 | Forward 5'-TGTGCAGAAGCCATCTCAAG-3' | 99 |
| Â | Â | Reverse 5'-AGATCCAAGAGGGCCTGATT-3' | Â |
Opn | NM_009263 | Forward 5'-GACCATGAGATTGGCAGTGA-3' | 110 |
| Â | Â | Reverse 5'-GGTGCAGGCTGTAAAGCTTC-3' | Â |
Odc | NM_013614 | Forward 5'-TTGCCACTGATGATTCCAAA-3' | 129 |
| Â | Â | Reverse 5'-CATGGAAGCTCACACCAATG-3' | Â |
Calm2 | NM_007589 | Forward 5'-CATGGCTGACCAACTGACTG-3' | 103 |
| Â | Â | Reverse 5'-CCCCAGCTCCTTTGTTGTTA-3' | Â |
Igfbp7 | NM_008048 | Forward 5'-GCATCCAGCCACCTTATGAT-3' | 105 |
| Â | Â | Reverse 5'-ATCCGAAGAGGAGGAAGAGG-3' | Â |
Rps29 | NM_009093 | Forward 5'-ACGGTCTGATCCGCAAATAC-3' | 138 |
| Â | Â | Reverse 5'-CATGATCGGTTCCACTTGGT-3' | Â |
Tctp | NM_009429 | Forward 5'-CCGGGAGATCGCGGAC-3' | 92 |
| Â | Â | Reverse 5'-TTCCACCGATGAGCGAGTC-3' | Â |
b. Primers using Taq Man technology Assay ID (Assay On Demand, Applied Biosystems) | |||
Hprt | NM_013556 | Mm00446968m1 | Â |
Kap | NM_010594 | Mm00495104m1 | Â |
Umod | NM_009470 | Mm00447649m1 | Â |
NaPi-II | NM_011392 | Mm00441450m1 | Â |