Figure 3

Schematic representation of the 3-kb chromosomal region around cg0910 (blue arrow) in C. glutamicum. His^- and his⁺ denote a histidine-auxotrophic or -prototrophic phenotype of the corresponding mutant or strain, respectively. The transposon integration (A) is marked with a green flag. Red arrows indicate the binding positions of the primers used to amplify the deletion fragments (B; yellow boxes). The features of the deletion- (B) and complementation (C) constructs are also designated. The ruler indicates the absolute position in the genome.