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Table 1 Target site analyses of randomly selected C. glutamicum transposon mutants.

From: Random mutagenesis in Corynebacterium glutamicum ATCC 13032 using an IS6100-based transposon vector identified the last unknown gene in the histidine biosynthesis pathway

Clone notation a

Integration position b

Locus tag c

Gene

Gene product

Target site duplication (TSD) d

G+C content [%] e

Transposon orientation f

51G06

141,831

cg0165

 

ABC-2 type transporter

GGCGCGCG

78.5

-

01A01

603,250

cg0683

 

Permease

AGTGAACC

53.5

+

52D11

737,930

cg0822

 

Conserved hypothetical protein

CCCAACGG

60.7

+

51B11

853,795

cg0924

 

ABC-type cobalamin/Fe3+-siderophores transport system periplasmic components

CTCAACGG

53.6

+

31D11

899,182

cg0963_0964

  

CTCTTTTT

39.3

+

51H01

1,097,334

cg1185

tnp10b

Transposase – fragment ISCg10a

AAAAATAC

57.1

+

51D02

1,099,353

cg1192

 

Aldo/keto reductase

CCCTAGCG

46.5

+

147A01

1,129,669

cg1228

 

ABC-type cobalt transport system, ATPase component

CCTACGTT

42.9

+

51B03

1,137,471

cg1237

 

Putative membrane protein

CCTTTGAG

42.9

+

51E04

1,161,219

cg1265

 

Conserved hypothetical protein

TAAGGAAG

39.2

-

51E02

1,217,257

cg1310

tfdF

Maleylacetate reductase

CGATTACG

50.0

-

51G12

1,246,055

cg1338

thrB

Homoserine Kinase

CCTATTAC

46.4

-

57D08

1,408,925

cg1516

 

Hypothetical protein

GCGATATC

46.4

-

121D05

1,613,084

cg1724

 

Putative protein kinase ArgK or related GTPase of G3E family

AGGTGAAG

60.7

+

43C10

1,717,632

cg1825

efp

Translation elongation factor P

CGGGTGTC

60.7

+

157H05

1,944,316

cg2053_2054

 

upstream putative membrane protein (cg2054)

CTTGATTC

42.9

+

29F07

2,180,143

cg2296

hisI

Probable phosphoribosyl-AMP cyclohydrolase

CGCCAAAG

57.1

-

17B06

2,234,105

cg2349

 

ATPase components of ABC transporters with duplicated ATPase domains

TTCTGGAA

64.3

-

75E09

2,418,388

cg2537

brnQ

Branched-chain amino acid uptake carrier

GTTTCATT

42.8

+

51C02

2,537,800

cg2661

 

Putative dithiol-disulfide isomerase involved in polyketide biosynthesis

ACTGGACT

53.6

+

51A01

2,772,437

cg2913

 

ABC-type Mn2+/Zn2+ transport system, permease component

TTGCTGAT

57.2

+

97B06

2,913,169

cg3049_3050

 

upstream acyltransferase (cg3050)

CTCGACTG

57.1

+

02A09

2,958,982

cg3100

dnaK

Heat shock protein Hsp70

TTCTCAGC

53.5

+

51A02

3,132,229

cg3271_3272

  

GTCAGCCG

60.7

-

35E03

3,166,513

cg3319_3320

 

upstream uncharacterized enzyme related to sulfurtransferases (cg3319)

CTTCAGTC

46.4

+

51F01

3,222,657

cg3373

 

Bacterial regulatory protein, ArsR family

CTTCCGCG

60.8

+

  1. a Indicates the position of the clone in the transposon library.
  2. b Absolute position of the cointegrate in the C. glutamicum ATCC 13032 genome [GenBank:BX927147].
  3. c Integration of a transposon within or between genes (separated by underscoring)
  4. d Sequence of the 8-bp direct repeat.
  5. e G+C content was calculated from 28 nucleotides around integration (TSD and 10 nt down-/upstream).
  6. f Orientation of the integrated transposon with respect to the genome in clockwise (-) and counterclockwise (+) direction
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BMC Genomics

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