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Table 1 Mycelium up-regulated genes identified by in silico ESTs subtraction and cDNA microarray.

From: Cell organisation, sulphur metabolism and ion transport-related genes are differentially expressed in Paracoccidioides brasiliensis mycelium and yeast cells

PbAEST

Acession Numbers (GenBank)

Annotated function

Number of reads

P-valuea

Fold change

Accession Number/Best-hit organism/E-value

Functional categories

   

M

Y

    

202

CA582032

1,3-beta-glucosidase*

7

2

0.036942

12.3

AAL09828.1/C. immitis/1.0E-132

Control of cell organization: Cell wall and membrane

2155

CA582352

Peroxisomal membrane protein PEX16 (peroxin-16)

7

0

0.004174

1.4

EAL88469.1/A. fumigatus/3.0E-64

 

186

CA583085

HEX*

13

8

0.049272

3.4

EAL91716.1/A. fumigatus/3.0E-66

 

2496

CA583518

Iron-sulphur cluster nifU-like protein*

5

1

0.048854

1.7

EAL90111.1/A. fumigatus/8.0E-58

Ion transport

4179

CN245816

Potassium transporter protein*

0

1

-b

5.2

CAA08814.1/N. crassa/4.0E-22

 

1420

CN247275

U1 small nuclear ribonucleoprotein

9

1

0.00526

1.6

EAL91268.1/A. fumigatus/1.0E-60

Transcription

1029

CA582332

Methyltransferase

32

1

0.000000

2.1

EAL84975.1/A. fumigatus/1.0E-56

Others

2096

CA581148

Unkown

20

1

0.000006

5.6

-

 

514

CA583322

Unkown

15

1

0.000138

23.4

-

 

1045

CA581951

Unkown

13

2

0.001769

24

-

 

1178

CN247241

Unkown

10

0

0.000535

8.5

-

 

1664

CN247289

Unkown

10

3

0.018648

2.5

-

 
  1. aFDR = 4,8% and Q-value < 5%.
  2. bNot significant by Audic-Claverie's method.
  3. * Up-regulated genes confirmed by northern blotting.
  4. ** Not assayed by cDNA microarray but confirmed as up-regulated in mycelium by northern blotting.
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BMC Genomics

ISSN: 1471-2164

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