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Table 2 Yeast up-regulated genes identified by in silico ESTs subtraction and cDNA microarray.

From: Cell organisation, sulphur metabolism and ion transport-related genes are differentially expressed in Paracoccidioides brasiliensis mycelium and yeast cells

PbAEST Acession Numbers (GenBank) Annotated function Number of reads P-valuea Fold change Accession Number/Best-hit organism/E-value Functional categories
    M Y     
1422 CA581980 Alpha-1,2-mannosyltransferase (Alg11) 4 11 0.019803 2.0 EAL88400.1/A. fumigatus/1.0E-130 Control of cellular organization: Cell wall and membrane
4988 CN253911 Alpha 1,3-glucan synthase* - 1 - 5.7 AAV52833.1/P brasiliensis/4.0E-93  
2162 CN238153 Putative WW domain protein (probable membrane protein) 4 12 0.013092 3.6 EAL85876.1/A. fumigatus/6.0E-17  
136 CA582283 Involved in cytoskeletal organization and cellular growth (verprolin)* 4 10 0.029289 4.0 NP_013441.1/S. cerevisiae/2.3  
667 CA583397 Adenylylsulphate kinase 3 8 0.038949 2.1 EAL90409.1/A. fumigatus/1.0E-88 Ion transport and metabolism
48 CA582091 ATP-sulphurylase 10 18 0.023038 4.8 EAL92915.1/A. fumigatus/0.0  
2031 CA581274 Outer mitochondrial membrane protein porin 1 14 0.000207 1.3 XP_323644.1/N. crassa/1.0E-108  
2724 CA581633 P-type Cu(2+) transporting ATPase* 0 1 -b 3.8 NP_009854.1/S. cerevisiae/1.7E-20  
635 CN247312 ATP citrate lyase 1 7 0.014984 1.9 EAL88915.1/A. fumigatus/0.0 Energy
2016 CN242578 ATPase inhibitor; Inh1 2 14 0.000835 2.7 NP_010100.1/S. cerevisiae/4.0E-08  
563 CA583982 Cytochrome c oxidase subunit VII 11 43 0.000002 2.1 AAT77147.1/P. brasiliensis/3.0E-26  
2398 CN240705 Disulfide isomerase 3 8 0.038949 2.1 EAL91387.1/A.fumigatus/3.0E-61  
540 CN240558 Cytochrome C oxidase biogenesis protein 0 5 0.015111 1.8 XP_214182.2/R. norvegicus/1.0E-06  
578 CA582837 Pyruvate dehydrogenase e1 component beta subunit 2 7 0.033994 1.6 EAL86696.1/A. fumigatus/2.0E-99  
407 CA583387 Succinyl-CoA synthetase alpha subunit 7 19 0.004468 2.6 EAL91981.1/A. fumigatus/1.0E-155  
284 CN239025 Ubiquinol-cytochrome C reductase complex ubiquinonE-binding protein QP-C 0 4 0.030475 1.5 EAL90680.1/A. fumigatus/7.0E-29  
378 CA580847 Argininosuccinate synthase 0 6 0.007492 1.7 NP_229577.1/T. maritime/4.0E-77 Amino acid metabolism and transport
1618 CA583639 Aromatic-L-amino-acid decarboxylase 1 33 0.00000 17.2 EAL86509.1/A. fumigatus/0.0  
125 CA583825 Glycine cleavage system h protein 4 9 0.042192 1.4 EAL90537.1/A. fumigatus/6.0E-36  
1674 CA583874 Aldolase 5 14 0.010368 37.9 AAL34519.2/P. brasiliensis/0.0 C-compound and carbohydrate metabolism
42 CA581699 Phosphoglycerate kinase 1 10 0.002512 2.6 EAL90363.1/A. fumigatus/0.0  
9 CA581893 Beta-ketoacyl synthase (Cem 1) 1 5 0.045709 2.2 EAL87667.1/A. fumigatus/1.0E-88 Lipid, fatty-acid and isoprenoid metabolism
780 CA581145 GPR/FUN34 family protein 0 11 0.000225 14.9 EAL87502.1/A. fumigatus/6.0E-67  
1989 CA581550 Acetyl-coenzyme A synthetase (AcetatE – CoA ligase) (Acyl-activating enzyme) 1 9 0.004605 2.0 EAL89682.1/A. fumigatus/0.0  
1550 CA582818 NADH-cytochrome b5 reductase 0 6 0.007492 5.4 EAL88164.1/A. fumigatus/1.0E-86  
300 CA581937 Nucleoside diphosphate kinase 6 58 0.00000 1.6 AAP85295.1/A. fumigatus/2.0E-67 Nucleotide metabolism
547 CA583473 6,7-dimethyl-8-ribityllumazine synthase 0 6 0.007492 1.4 AAD55372.1/A. fumigatus/9.0E-56 Metabolism of vitamins, cofactors, and prosthetic groups
924 CN240624 Coproporphyrinogen III oxidase 2 7 0.033994 2.7 EAL88456.1/A. fumigatus/0.0  
867 CA580742 NADH pyrophosphatase 1 5 0.045709 5.7 EAL85969.1/A. fumigatus/1.0E-159  
1490 CA583063 Pyridoxamine 5'-phosphate oxidase 0 10 0.000453 3.5 AAC28862.1/S. commune/2.0E-32  
447 CA580589 NADH:ubiquinone oxidoreductase B18 subunit 1 10 0.002512 1.4 EAL92195.1/A. fumigatus/9.0E-33  
488 CA582788 Exonuclease II 1 5 0.045709 1.9 EAL85993.1/A. fumigatus/1.0E-138 Transcription, translation and ribosome structure
165 CN241393 RNP domain protein 3 13 0.003962 1.8 EAL89070.1/A. fumigatus/5.0E-81  
2436 CA580512 Splicing factor u2af 35 kd subunit 2 7 0.033994 2.5 EAL86523.1/A. fumigatus/1.0E-103  
253 CN240426 Zinc finger, C3HC4 type 0 5 0.015111 1.4 NP_593329.1/S. cerevisiae/3.0E-10  
551 CN239696 Ribosomal protein L35** 5 10 0.044755 - AAL08563.1/P. brasiliensis/1.0E-63  
979 CA582579 60S ribosomal protein L7/L12 precursor 1 8 0.008358 1.3 EAL89813.1/A. fumigatus/4.0E-49  
175 CA581863 Complex I intermediatE-associated protein CIA30 precursor 4 15 0.003399 5.6 EAL92946.1/A. fumigatus/1.0E-114 Protein fate and Secretion
832 CN242383 Glutathione S transferase 1 7 0.014984 2.0 NP_588171.1/S. pombe/7.0E-42  
2387 CA584103 Non-classical export protein (Nce1) 1 7 0.014984 55.6 EAL87256.1/A. fumigatus/1.0E-29  
1823 CA583903 Profilin 1 5 0.045709 1.3 NP_014765.1/S. cerevisiae/8.0E-14  
4188 CN245872 Mating type protein (MAT1–2)* 1 0 - 8.0 EAL89707.1/A. fumigatus/2.0E-36 Mating Type
50 CA581392 Cu-Zn superoxide dismutasE-related* 0 8 0.001842 2.1 CAB97297.1/N. crassa/3.0E-30 Virulence and oxidative stress
2059 CN241260 Ribosome associated protein (Stm1) 6 31 0.000007 1.7 EAL92489.1/A. fumigatus/2.0E-32 Others
2005 CA580764 Signal peptide protein 1 6 0.026442 2.3 EAL93249.1/A. fumigatus/7.0E-68  
39 CA581046 Unknown 0 6 0.007492 2.2 -  
33 CA582496 Unknown 0 8 0.001842 3.1 -  
1442 CA581846 Unknown 3 16 0.000836 4.5 -  
2399 CA581839 Unknown 1 5 0.045709 2.5 -  
512 CA583749 Unknown 0 6 0.007492 4.3 -  
639 CA581506 Unknown 0 7 0.003715 1.7 -  
718 CN247671 Unknown 0 6 0.007492 1.8 -  
765 CA581478 Unknown 0 10 0.000453 3.9 -  
529 CA580398 Unknown 1 5 0.045709 18.8 -  
  1. aFDR = 4,8% and Q-value < 5%.
  2. bNot significant by Audic-Claverie's method.
  3. * Up-regulated genes confirmed by northern blotting.
  4. ** Not assayed by cDNA microarray but confirmed as up-regulated in yeast by northern blotting.