Table 1 Differentiated, human cord blood-derived, mast cells were sensitized overnight by the addition of monomeric IgE followed by crosslinking the high affinity IgE receptors, FcεRI using anti IgE for 2 h, 6 h and 12 h. Gene expression profile at each time point were analyzed and 115 genes, which were upregulated 2 fold or more (over control/unstimulated cells) atleast in one point have been selected and classified according to their biological functions as a) Cytokines and cytokine receptors b) Chemokine and chemokine receptors c) Other immunoregulatory genes d)Cell proliferation and anti-apoptosis e)Adhesion and cytoskeleton remodeling f)Transcription factors and regulators g) Signal transduction h) Other genes. Fold change is expressed as fold-increase over control/unsensitized samples, and representative of four separate experiments.
a. Cytokines and cytokine receptors | ||||||
|---|---|---|---|---|---|---|
Genbank ID | Gene symbol | Gene name | IgE Sens | 2 h | 6 h | 12 h |
M15330 | IL1B | Interleukin 1, beta | 3.3 | 4.8 | 1.5 | -2.0 |
NM_000600.1 | IL6 | Interleukin 6 | 2.0 | 2.6 | 1.9 | -1.3 |
M37435 | CSF1 | Colony stimulating factor 1 | 1.6 | 4.5 | 1.9 | -1.4 |
NM_000877.1 | IL1R1 | Interleukin 1 receptor, type I | 1.4 | 2.2 | 1.9 | -1.2 |
AI983115 | IL27RA | Class I cytokine receptor | 1.4 | 1.3 | 2.6 | 1.1 |
NM_001561.2 | TNFRSF9 | Tumor necrosis factor receptor superfamily, member 9 | 1.2 | 1.5 | 2.5 | 1.7 |
NM_016639.1 | TNFRSF12A | Tumor necrosis factor receptor superfamily, member 12A | 1.5 | 2.4 | -1.3 | -1.9 |
X52015 | IL1RN | Interleukin 1 receptor antagonist | 1.1 | 2.4 | 2.6 | 1.4 |
b. Chemokine and chemokine receptors | ||||||
Genbank ID | Gene symbol | Gene name | IgE Sens | 2 h | 6 h | 12 h |
NM_002984.1 | CCL4 | Chemokine (C-C motif) ligand 4 | 3.5 | 4.7 | 1.6 | -3.0 |
NM_001511.1 | CXCL1 | Chemokine (C-X-C motif) ligand 1 | 2.3 | 2.5 | 1.1 | -2.9 |
NM_006273.2 | CCL7 | Chemokine (C-C motif) ligand 7 | 2.1 | 3.6 | 5.3 | 2.2 |
NM_002090.1 | CXCL3 | Chemokine (C-X-C motif) ligand 3 | 1.6 | 2.5 | 1.6 | -1.8 |
NM_002985.1 | CCL5 | Chemokine (C-C motif) ligand 5 | 1.6 | 1.8 | 2.1 | 1.8 |
AF015524 | CCRL2 | Chemokine (C-C motif) receptor-like 2 | 1.5 | 2.0 | 2.0 | 1.2 |
AF043337 | IL8 | Interleukin 8 | 2.1 | 2.1 | 1.5 | -1.5 |
c. Other immunoregulatory genes | ||||||
Genbank ID | Gene symbol | Gene name | IgE Sens | 2 h | 6 h | 12 h |
S59049 | BL34 | B cell activation gene | 3.4 | 6.8 | -2.3 | -3.9 |
NM_002309.2 | LIF | Leukemia inhibitory factor (Cholinergic differentiation factor) | -1.3 | 5.1 | -1.3 | -4.0 |
L07555 | CD69 | CD69 antigen | -1.1 | 4.1 | -1.0 | -1.7 |
AF036906 | LAT | Linker for activation of T cells | 1.9 | 2.3 | 2.3 | 1.7 |
NM_004233.1 | CD83 | CD83 antigen | 2.4 | 3.0 | 1.6 | -1.2 |
NM_000675.2 | ADORA2A | Adenosine A2a receptor | 2.1 | 3.8 | 1.4 | -1.3 |
NM_001311.1 | CRIP1 | Cysteine-rich protein 1 | 1.5 | 2.6 | 2.7 | 1.3 |
M16276 | HLA-DQB1 | Major histocompatibility complex, class II, DQ beta 1 | 1.9 | 2.0 | 1.7 | 1.2 |
AW007185 | HKE2 | HLA class II region expressed gene KE2 | 1.4 | 1.4 | 2.2 | 1.9 |
NM_005658.1 | TRAF1 | TNF receptor-associated factor 1 | 1.5 | 2.5 | 1.8 | -1.0 |
NM_005601 | NKG7 | Natural killer cell group 7 sequence | 1.4 | 2.2 | 1.3 | 1.3 |
NM_002852.1 | PTX3 | Pentaxin-related gene, rapidly induced by IL-1 beta | 1.8 | 2.5 | 1.5 | -2.1 |
M31933 | FCGR2B | Fc fragment of IgG, low affinity IIb, receptor for (CD32) | 2.0 | 2.0 | 1.3 | -1.1 |
NM_007115.1 | TNFAIP6 | Tumor necrosis factor, alpha-induced protein 6 | 2.0 | 2.4 | 2.1 | -1.1 |
NM_000956.1 | PTGER2 | Prostaglandin E receptor 2 (subtype EP2) | 2.0 | 2.2 | 1.3 | -1.5 |
NM_013252.1 | CLECSF5 | C-type lectin, superfamily member 5 | 1.6 | 2.3 | 2.5 | 1.2 |
NM_004951.1 | EBI2 | Epstein-Barr virus induced gene 2 (lymphocyte-specific GPCR) | 1.4 | 2.0 | -1.7 | -2.0 |
NM_018643.1 | TREM1 | Triggering receptor expressed on myeloid cells 1 | 1.4 | 2.2 | 2.5 | 1.7 |
U37546 | BIRC3 | Baculoviral IAP repeat-containing 3 | 1.4 | 2.0 | 1.1 | -1.1 |
NM_002000.1 | FCAR | Fc fragment of IgA, receptor for | 1.4 | 2.0 | 1.7 | -1.9 |
NM_003264.1 | TLR2 | Toll-like receptor 2 | 1.4 | 2.0 | 2.0 | -1.6 |
NM_002114.1 | HIVEP1 | Human immunodeficiency virus type I enhancer binding protein 1 | 2.0 | 1.6 | -1.2 | -1.6 |
d. Cell proliferation and anti-apoptosis | ||||||
Genbank ID | Gene symbol | Gene name | IgE Sens | 2 h | 6 h | 12 h |
NM_006732.1 | FOSB | FBJ murine osteosarcoma viral oncogene homolog B | 2.1 | 3.9 | -3.0 | -4.9 |
NM_004430.1 | EGR3 | Early growth response 3 | 1.8 | 7.5 | -1.4 | -3.3 |
AF001294 | PHLDA2 | Tumor suppressing subtransferable candidate 3 | 2.6 | 3.8 | 1.7 | -1.2 |
NM_002575.1 | SERPINB2 | Serine (or cysteine) proteinase inhibitor, clade B (ovalbumin), member 2 | 1.9 | 3.8 | 5.2 | -1.1 |
M60485 | FGFR1 | Ffibroblast growth factor receptor 1 (fms-related tyrosine kinase 2, Pfeiffer syndrome) | 2.3 | 2.2 | 2.7 | 1.5 |
NM_000399.2 | EGR2 | Early growth response 2 (Krox-20 homolog, Drosophila) | 1.6 | 2.7 | 1.6 | -1.9 |
NM_005542.3 | INSIG1 | Insulin induced gene 1 | 1.7 | 2.8 | 1.6 | -1.2 |
NM_002607.1 | PDGFA | Platelet-derived growth factor alpha polypeptide | 1.8 | 2.4 | 1.7 | 1.1 |
NM_003897.1 | IER3 | Immediate early response 3 | 1.8 | 3.0 | -1.2 | -2.3 |
NM_002608.1 | PDGFB | Platelet-derived growth factor beta polypeptide | 1.2 | 2.0 | -1.2 | 1.0 |
NM_006763.1 | BTG2 | BTG family, member 2 | 1.5 | 2.0 | -1.1 | -1.9 |
NM_005655.1 | TIEG | TGFB inducible early growth response | 1.8 | 1.9 | -1.8 | -2.4 |
NM_004073.1 | CNK | Cytokine-inducible kinase | 1.2 | 1.8 | -1.5 | -2.4 |
BF575514 | PBEF1 | Pre-B-cell colony-enhancing factor | 1.6 | 1.9 | -1.3 | -3.2 |
NM_006290.1 | TNFAIP3 | TNF-induced protein 3 | 2.7 | 3.4 | 1.8 | -1.0 |
BC005352 | TNFAIP8 | TNF-induced protein 8 | 2.0 | 1.6 | 1.3 | 1.3 |
NM_023009.1 | MLP | MARCKS-like protein | 1.5 | 1.7 | 2.5 | 1.1 |
NM_001878.2 | CRABP2 | Cellular retinoic acid binding protein 2 | 1.1 | 1.2 | 1.4 | 2.0 |
NM_002199.2 | IRF2 | Interferon regulatory factor 2 | -1.5 | -1.7 | 1.2 | 2.0 |
e. Adhesion and cytoskeleton remodeling | ||||||
Genbank ID | Gene symbol | Gene name | IgE Sens | 2 h | 6 h | 12 h |
NM_004658.1 | RASAL1 | RAS protein activator like 1 (GAP1 like) | 2.2 | 2.2 | 3.6 | 2.3 |
NM_013231.1 | FLRT2 | Fibronectin leucine rich transmembrane protein 2 | 1.4 | 2.3 | 2.3 | 2.4 |
NM_003078.1 | SMARCD3 | SWI/SNF related, matrix associated, actin dependent regulator of chromatin, subfamily d, member 3 | 2.2 | 1.9 | 1.2 | 1.1 |
BG054844 | ARHE | Ras homolog gene family, member E | 1.3 | 2.2 | 1.9 | -1.0 |
NM_000216.1 | KAL1 | Kallmann syndrome 1 sequence | 1.4 | 1.4 | 1.3 | 2.0 |
M62994 | FLNB | Filamin B, beta (actin binding protein 278) | 1.2 | 1.4 | 2.0 | 1.0 |
NM_004357.1 | CD151 | CD151 antigen | 1.0 | 1.2 | 2.1 | -1.1 |
AA243143 | ARF6 | ADP-ribosylation factor 6 | 2.1 | 1.3 | 1.1 | 1.0 |
NM_001627.2 | ALCAM | Activated leukocyte cell adhesion molecule | 1.1 | 1.1 | 1.7 | 2.0 |
f. Transcription factors and regulators | ||||||
Genbank ID | Gene symbol | Gene name | IgE Sens | 2 h | 6 h | 12 h |
AL021977 | MAFF | V-maf musculoaponeurotic fibrosarcoma oncogene homolog F (avian) | 2.6 | 6.3 | 1.2 | -2.2 |
AF060154 | MSC | Musculin (activated B-cell factor-1) | 1.8 | 2.4 | 1.9 | 1.1 |
M55643 | NFKB1 | Nuclear factor of kappa light polypeptide gene enhancer in B-cells 1 (p105) | 1.7 | 2.1 | 1.5 | -1.0 |
NM_001706.1 | BCL6 | B-cell CLL/lymphoma 6 | 2.4 | 2.0 | 1.5 | 1.2 |
U08015 | NFATC1 | Nuclear factor of activated T-cells, cytoplasmic, calcineurin-dependent 1 | 1.3 | 2.3 | -1.1 | -1.1 |
NM_001674.2 | ATF3 | Activating transcription factor 3 | 1.3 | 2.0 | -1.0 | -1.6 |
AI078167 | NFKBIA | Nuclear factor of kappa light polypeptide gene enhancer in B-cells inhibitor, alpha | 1.9 | 2.0 | 1.3 | -1.5 |
NM_004556.1 | NFKBIE | Nuclear factor of kappa light polypeptide gene enhancer in B-cells inhibitor, epsilon | 1.7 | 2.0 | 1.1 | -1.2 |
NM_002467.1 | MYC | V-myc myelocytomatosis viral oncogene homolog (avian) | 1.7 | 2.1 | 1.1 | -1.3 |
NM_001423.1 | EMP1 | Epithelial membrane protein 1 | 1.4 | 1.6 | 2.1 | 1.3 |
NM_012081 | ELL2 | ELL-related RNA polymerase II, elongation factor | 1.7 | 1.3 | 2.6 | 1.3 |
NM_001067.2 | TOP2A | Topoisomerase (DNA) II alpha 170kDa | 2.0 | 1.0 | 1.2 | 1.3 |
AF078847 | GTF2H2 | General transcription factor IIH, polypeptide 2, 44kDa | -1.0 | 1.1 | 2.0 | -1.1 |
g. Signal transduction | ||||||
Genbank ID | Gene symbol | Gene name | IgE Sens | 2 h | 6 h | 12 h |
NM_004418.2 | DUSP2 | Dual specificity phosphatase 2 | 4.3 | 7.2 | 1.8 | 1.5 |
NM_000361.1 | THBD | Thrombomodulin | 2.5 | 4.5 | 1.0 | -1.7 |
S77154 | NR4A2 | Nuclear receptor subfamily 4, group A, member 2 | 1.3 | 4.1 | -1.0 | -2.1 |
NM_005261.1 | GEM | GTP binding protein overexpressed in skeletal muscle | 1.2 | 2.7 | 1.1 | -1.1 |
AF035776 | OLR1 | Oxidised low density lipoprotein (lectin-like) receptor 1 | 2.0 | 2.6 | 3.1 | -1.0 |
NM_000161.1 | GCH1 | GTP cyclohydrolase 1 (dopa-responsive dystonia) | 2.0 | 2.4 | 1.7 | -1.9 |
NM_021972.1 | SPHK1 | Sphingosine kinase 1 | 1.4 | 2.1 | 2.7 | -1.0 |
NM_005001.1 | NDUFA7 | NADH dehydrogenase (ubiquinone) 1 alpha subcomplex, 7, 14.5kDa | 1.6 | 1.6 | 2.0 | 1.4 |
M60278 | HBEGF | Heparin-binding EGF-like growth factor | 1.1 | 2.4 | -2.1 | -5.0 |
U77914 | JAG1 | Jagged 1 (Alagille syndrome) | 1.4 | 2.5 | 1.8 | 1.0 |
NM_000527.2 | LDLR | Low density lipoprotein receptor | 1.6 | 2.0 | -1.8 | -1.4 |
NM_005904.1 | MADH7 | MAD, mothers against decapentaplegic homolog 7 (Drosophila) | 1.7 | 1.9 | -1.2 | -2.0 |
AF070528 | MALT1 | Mucosa associated lymphoid tissue lymphoma translocation gene 1 | 1.5 | 2.1 | 1.2 | -1.2 |
NM_005842.1 | SPRY2 | Sprouty homolog 2 (Drosophila) | 1.1 | 2.2 | 1.4 | -1.3 |
BC003143 | DUSP6 | Dual specificity phosphatase 6 | 1.2 | 2.2 | 1.2 | -1.4 |
AA780381 | MAP2K3 | Mitogen-activated protein kinase kinase 3 | -1.0 | 2.1 | -1.0 | -1.7 |
NM_001881.1 | CREM | cAMP responsive element modulator | 1.2 | 2.0 | -1.0 | -1.1 |
NM_004417.2 | DUSP1 | Dual specificity phosphatase 1 | 1.6 | 1.8 | -2.3 | -3.3 |
NM_003954.1 | MAP3K14 | Mitogen-activated protein kinase kinase kinase 14 | 1.5 | 2.0 | 1.3 | 1.4 |
AF305083 | FUT4 | Fucosyltransferase 4 (alpha (1,3) fucosyltransferase, myeloid-specific) | 2.0 | 1.6 | 1.2 | -1.5 |
BE327172 | JUN | V-jun sarcoma virus 17 oncogene homolog | 1.7 | 1.5 | 1.4 | 2.0 |
L06633 | PSCDBP | Pleckstrin homology, Sec7 and coiled/coil domains, binding protein | 1.3 | 1.6 | 2.0 | 1.1 |
M14333 | FYN | FYN oncogene related to SRC, FGR, YES | 1.3 | 1.5 | 3.7 | 1.6 |
NM_005023.1 | PGGT1B | Protein geranylgeranyltransferase type I, beta subunit | 1.7 | 1.4 | 1.5 | 2.1 |
NM_006296.1 | VRK2 | Vaccinia related kinase 2 | 1.3 | 1.4 | 2.0 | -1.1 |
AF127481 | proto-LBC | A kinase (PRKA) anchor protein 13 | 1.4 | -1.1 | 1.3 | 2.0 |
NM_003318.1 | TTK | TTK protein kinase | 1.6 | -1.1 | 2.1 | 1.6 |
h. Other genes | ||||||
Genbank ID | Gene symbol | Gene name | IgE Sens | 2 h | 6 h | 12 h |
NM_003633.1 | ENC1 | Ectodermal-neural cortex (with BTB-like domain) | 2.2 | 3.1 | 1.1 | 1.4 |
NM_004694.1 | SLC16A6 | Solute carrier family 16 (monocarboxylic acid transporters), member 6 | 2.6 | 2.5 | 1.0 | 1.4 |
AA451996 | HIST2H2AA | H2A histone family, member O | 2.3 | 2.4 | 2.4 | 1.4 |
AF182273 | CYP3A4 | Cytochrome P450, subfamily IIIA (niphedipine oxidase), polypeptide 4 | 1.2 | 2.0 | 1.0 | 1.1 |
BC002649 | HIST1H1C | Histone 1, Hic | 2.1 | 1.8 | 2.0 | 1.7 |
NM_006101.1 | HEC | Highly expressed in cancer, rich in leucine heptad repeats | 2.3 | 1.3 | 2.1 | 1.6 |
NM_004760.1 | STK17A | Serine/threonine kinase 17a | 1.4 | 1.8 | 2.0 | -1.1 |
NM_020651.2 | PELI1 | Pellino homolog 1 (Drosophila) | 2.2 | 1.4 | 1.4 | 1.0 |
L39833 | KCNAB1 | Potassium voltage-gated channel, shaker-related subfamily, beta member 1 | 2.4 | 1.4 | 1.1 | 1.4 |
BF223021 | B4GALT4 | UDP-Gal:betaGlcNAc beta 1,4-galactosyltransferase, polypeptide 4 | 2.0 | 1.2 | 1.5 | 1.2 |