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Table 2 Comparison of Halobacterium GC coverage. Mean sequence GC contents, standard deviations, P-values and 95% Confidence limits around differences between the test and unamplified control population means for Halobacterium species NRC-1.

From: Assessment of whole genome amplification-induced bias through high-throughput, massively parallel whole genome sequencing

Population

P Value

Unamplified Control Mean GC Content

Test Population Mean GC Content

Lower 95% Confidence Limit

Upper 95% Confidence Limit

Unamplified Replicate 1

0.32

61.21 ± 8.6%

61.25 ± 8.6%

-0.11%

0.04%

Unamplified Replicate 2

0.88

 

61.21 ± 8.6%

-0.07%

0.08%

Unamplified Replicate 3

0.92

 

61.21 ± 8.6%

-0.07%

0.08%

Unamplified Replicate 4

0.23

 

61.26 ± 8.6%

-0.12%

0.03%

GenomiPhi

< 0.001

 

58.29 ± 7.7%

2.85%

2.99%

Repli-G

< 0.001

 

57.36 ± 7.4%

3.78%

3.92%

PEP-PCR

< 0.001

 

56.25 ± 7.2%

4.90%

5.04%

DOP-PCR

< 0.001

 

55.20 ± 7.0%

5.94%

6.08%