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Table 3 Comparison of Campylobacter jejuni GC coverage. Mean sequence GC contents, standard deviations, P-values and 95% Confidence limits around differences between the test and unamplified reference population means for Campylobacter jejuni.

From: Assessment of whole genome amplification-induced bias through high-throughput, massively parallel whole genome sequencing

Population

P Value

Unamplified Control Mean GC Content

Test Population Mean GC Content

Lower 95% Confidence Limit

Upper 95% Confidence Limit

Unamplified Replicate 1

0.65

31.15 ± 6.7%

31.16 ± 6.7%

-0.07%

0.05%

Unamplified Replicate 2

0.75

 

31.16 ± 6.7%

-0.07%

0.05%

Unamplified Replicate 3

0.83

 

31.15 ± 6.7%

-0.07%

0.05%

Unamplified Replicate 4

0.75

 

31.16 ± 6.7%

-0.07%

0.05%

GenomiPhi

< 0.001

 

31.36 ± 6.8%

-0.28%

-0.16%

Repli-G

< 0.001

 

31.60 ± 6.7%

-0.52%

-0.40%

PEP-PCR

< 0.001

 

33.92 ± 8.1%

-2.84%

-2.71%

DOP-PCR

< 0.001

 

30.79 ± 5.9%

0.30%

0.41%