Table 5 Raw differences in counts per bin between control and test populations. Numbers in parenthesis are the ratio of the WGA amplified samples normalized to the average deviation in the unamplified replicate populations.
Halobacterium – 100 base bins | ||||||||
|---|---|---|---|---|---|---|---|---|
Versus Unamplified Control | Replicate 1 | Replicate 2 | Replicate 3 | Replicate 4 | GenomiPhi | Repli-G | PEP-PCR | DOP-PCR |
Maximum Fold Overamplification | 10 | 10 | 14 | 11 | 43 (3.8) | 69 (6.1) | 299 (26.6) | 1633 (145.2) |
Maximum Fold Underamplification | 11 | 12 | 10 | 11 | 17 (1.5) | 17 (1.5) | 24 (2.2) | 74 (6.7) |
Maximum Difference in Counts per bin | 27 | 26 | 31 | 35 | 67 | 101 | 299 | 1633 |
Campylobacter – 100 base bins | ||||||||
Versus Unamplified Control | Replicate 1 | Replicate 2 | Replicate 3 | Replicate 4 | GenomiPhi | Repli-G | PEP-PCR | DOP-PCR |
Maximum Fold Overamplification | 13 | 13 | 13 | 14 | 16 (1.2) | 23 (1.7) | 31 (2.3) | 2668.5 (201.4) |
Maximum Fold Underamplification | 11 | 15 | 12 | 14 | 15 (1.2) | 16 (1.2) | 19 (1.5) | 54 (4.2) |
Maximum Difference in Counts per bin | 18 | 17 | 15 | 17 | 23 | 23 | 6251 | 133 |
Halobacterium – 10 base bins | ||||||||
Versus Unamplified Control | Replicate 1 | Replicate 2 | Replicate 3 | Replicate 4 | GenomiPhi | Repli-G | PEP-PCR | DOP-PCR |
Maximum Fold Overamplification | 11 | 11 | 11 | 11 | 26 (2.4) | 23 (2.1) | 52 (4.7) | 458 (41.6) |
Maximum Fold Underamplification | 10 | 9 | 9 | 12 | 15 (1.5) | 15 (1.5) | 15 (1.5) | 15 (1.5) |
Maximum Difference in Counts per bin | 10 | 12 | 12 | 12 | 25 | 22 | 51 | 457 |
Campylobacter – 10 base bins | ||||||||
Versus Unamplified Control | Replicate 1 | Replicate 2 | Replicate 3 | Replicate 4 | GenomiPhi | Repli-G | PEP-PCR | DOP-PCR |
Maximum Fold Overamplification | 7 | 9 | 7 | 8 | 9 (1.2) | 14 (1.8) | 39 (5.0) | 991 (127.9) |
Maximum Fold Underamplification | 8 | 7 | 8 | 7 | 11 (1.5) | 11 (1.5) | 11 (1.5) | 11 (1.5) |
Maximum Difference in Counts per bin | 8 | 8 | 7 | 7 | 10 | 13 | 38 | 990 |