Table 6 Comparison of Halobacterium coverage bias. Kolmogorov-Smirnov comparison of the distributions of reads per bin from an unamplified sample of Halobacterium species NRC-1 with an additional unamplified replicate library and libraries amplified with GenomiPhi, Repli-G, PEP and DOP. Bin Size refers to the number of bases comprising each individual bin into which the genome was broken for analysis; 100,000 reads were used for each analysis. Ranked bias was derived from ranked D statistics, lowest to highest.
Unamplified Control versus: | Unamplified Replicate | GenomiPhi | RepliG | PEP | DOP |
|---|---|---|---|---|---|
Bin Size (bp) | 10 | ||||
Number of Bins | 257102 | ||||
Number of Reads | 100000 | ||||
D Statistic | 0.001 | 0.081 | 0.099 | 0.108 | 0.213 |
P value | 1.000 | 0.000 | 0.000 | 0.000 | 0.000 |
Ranked Bias (5 is lowest) | 5 | 4 | 3 | 2 | 1 |
Bin Size (bp) | 100 | ||||
Number of Bins | 25711 | ||||
Number of Reads | 100000 | ||||
D Statistic | 0.003 | 0.357 | 0.492 | 0.495 | 0.756 |
P value | 0.980 | 0.000 | 0.000 | 0.000 | 0.000 |
Ranked Bias (5 is lowest) | 5 | 4 | 3 | 2 | 1 |
Bin Size (bp) | 2571 | ||||
Number of Bins | 1001 | ||||
Number of Reads | 100000 | ||||
D Statistic | 0.028 | 0.648 | 0.717 | 0.736 | 0.837 |
P value | 0.791 | 0.000 | 0.000 | 0.000 | 0.000 |
Ranked Bias (5 is lowest) | 5 | 4 | 3 | 2 | 1 |
Bin Size (bp) | 25711 | ||||
Number of Bins | 100 | ||||
Number of Reads | 100000 | ||||
D Statistic | 0.095 | 0.630 | 0.650 | 0.660 | 0.720 |
P value | 0.737 | 0.000 | 0.000 | 0.000 | 0.000 |
Ranked Bias (5 is lowest) | 5 | 4 | 3 | 2 | 1 |