Table 1 Sequences of PtMPKs 3'RACE and RTqPCR primers used for 3'UTR MAPK gene isolation and expression profiling.
Group | Gene name | 3' RACE Primers (5' to 3') | Forward RTqPCR Primers (5' to 3') | Reverse RTqPCR Primers (5' to 3') |
|---|---|---|---|---|
A | PtMPK3-1 | CCCACTTGTTCACCCTCTGGCCATTG | CCATAAGCACTACCAGTCC | TCA GATACAGAGGAGTGC |
PtMPK3-2 | AGCATTGGCTCATCCATACCTTGCAAGG | CTTTAGCACTACCAATCCCG | TGAACAATTAGCAAGTCCAGG | |
PtMPK6-1 | TCCCAACTGTCCACCCGGCAGCTATTG | CATTGTGACTGGTCAGCTTGA | GTTCCCCTGAATTCTGTCC | |
PtMPK6-2 | GATCCCAGACAGAGGATTACTGTTGAGG | ATGAGTCGACCAGCGTGGC | CACTACATTGACGCCGATAC | |
B | PtMPK4 | AGTCGATGAGGCACTGTGCCATCCATAC | TGGTGTGCAGTTGGGAAGG | GAACCTTCATAGAGATTCTGC |
PtMPK5-1 | TCGCATCACTGTTGATGAGGCCCTTTGT | CGTCATTTCCTGGAGAATATG | ACAATGCAATTCACTTCAGGC | |
PtMPK5-2 | CCCAATAACCGCATCACTGTCGATGAGG | CATAACTTCTGACACATTTGGG | CATACAACACATGATCCTTGC | |
PtMPK11 | GAGGCATTGTGCCATCCATACTTGGCAC | GTTTGCTGAGCAGAATCTCC | GCGTCCTCAAAACCATGAAG | |
C | PtMPK1 | CCGGAGCACTGGAACACCCTTACATGTC | AAGAAGCACGCACAAGCCAG | TACAAGCTGAAAGGGCCACC |
PtMPK2 | GCTGCAACCCTCCAGCTCAGGTCCC | GTGTGTTTTTGTACGTGTCTG | AGAACTAGAAGCTCACTGCC | |
PtMPK7 | CAGGGCTGTACGATCCAAGACGCGACCC | CTAGTGTGTCTGTGAGATCAG | GGATGCCATAGTCCGGCTC | |
PtMPK14 | ACCCTTACATGTCAGGGCTGCACGATCC | TGCACGATCCAAGACACGAC | TGCGAAAACAACTTCTGGGTG | |
D | PtMPK9-1 | CCAGGGTCACAGCAACCAGATGGTTCAG | GATTCAGATGGCTGCCTTTG | ACAAGCTACTGCCTGATCTTC |
PtMPK9-2 | GCAGCTCAAAGCACTGATATTGAGAGGC | CCAGCGTAGTGTGGATAGTG | TCACATCTCCTCTCTGCAATC | |
PtMPK16-1 | CGGTGCTGCGTTACAACAATTGTGGAGC | AATCTGTGCCATTTTGTCTGTG | TGGCCTTATGAATACAGCCAC | |
PtMPK16-2 | GTGGAGCAGCAGCAGCAGAGAATCTTGA | CAGCAGCAGAGAATCTTGAC | CCATTGGAACCTTCAACCAC | |
PtMPK17 | CTCTTTCTCGTGCAGCTAAGCAGAGCCC | CCTATTTGCACATCTTGGAGG | GCGGTAGTACATTTAGTGAGG | |
PtMPK18 | CCTCCCCAAACCAGCTCTCCACACCACTGCT | GATAAATCCTTTCCACCCTCG | GTTGACTACACCCTTGAAACC | |
PtMPK19 | CTGGGATGGCCATGGATGTGAACGCCTA | GTTGGTATCACGATCAATTATG | TGACTAGATACAGGACGTGG | |
PtMPK20-1 | GGGAGGGAGCAAGGAAGAACATGTGGAT | GGACTGAGGAGATCCACTTG | CCCCCTCTTTTTACACAATCC | |
PtMPK20-2 | GTGGCATGGCAGCCAAATATGCACCAGA | GGTTGGAGCTGTTCAATATGG | GACACGTTGATCTTCTGAGTC |