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Table 2 Amplicons generated to represent 60 immunologically important genes.

From: Development and validation of a bovine macrophage specific cDNA microarray

Gene

Symbol

Accession No.

Size (bp)

Forward Primer 5'-3' sequence

Reverse Primer 5'-3' sequence

Annexin-A5

ANXA5

CO883855

700

ATG GCA CAG GTT CTC AGA GG

CCA AAT TGC CAG AAG TCT CC

Baculoviral IAP-repeat containing 5

BIRC5

BE486047

451

CCT GGC AGC TCT ACC TCA AG

CCC TGG AAA CGA CGT GTC TG

B-cell CLL/lymphoma 3

BCL3

CK771977

537

CTT CTT GGC ACT CGG TGT TC

GAC CAG AGG CAA TTT ACT ACC

Capping protein (actin filament) gelsolin like

CAPG

AY219899

615

TAT GGA GGG TGG AGA AGC TG

TGG GAC CCA AGA CCT GTA TC

Casein kinase II α1

CSNK2A1

X54962

405

GTT AAT ACA CAC AGA CCC CGA

GCT ATG GCA GTA ATC AAG GGC C

Caspase 3

CASP3

CK958902

679

GTG GAT GCA GCA AAC CTC AG

CCA TTT GCT CAC TTG GCA TAC

Caspase 7

CASP7

CK436554

549

TCT TCC AAA GTC ACC ACC AG

GGA GTG GCT CTT GGT TTG TG

Caspase recruitment domain family member 4

CARD4

CO886987

630

ATG TCG GAG CCA GGT ACA TC

GAA GTC CCA CCT CAG CAG AC

CD2 antigen

CD2

CN789613

544

CAG AAC TGT GAG CTG CAA GG

TTG CTG GTG AAC TTG TGT GC

CD3 antigen γ

CD3G

CD445323

518

GAA GGA GTT CGC CAG TCA AG

TGA TGA GAA TAG CCA ATA AGC AC

CD40 ligand

CD40LG

Z48469

652

TAA CGC AGC ATG ATC GAA AC

AGA ACT GTG GGT GTT TGC AG

CD63 antigen

CD63

AJ012589

606

TTC TCC TGC TGG TCT TCT GC

GGA TCT CCA CAA AGG CAA TG

CD80 antigen

CD80

BF039039

522

GGT ACT CCA ACC AGC GTC TC

AGG TGT AGG TGC CAC TGT CC

CD86 antigen

CD86

AJ291475

674

GCC TTC TTC AAC GAG ACT GG

GCA ATC CAG AGG ATG TGG TC

CD200 receptor 1

CD200R1

CK831048

552

CTG TGG ACA GAA AGC AGA GC

GGT TCT GAC ACC TTT ATC CAG

Chemokine (C motif) ligand 1

XCL1

AF354057

411

TCA GCC TCT TAC TGC ACA GC

AAA TGA GGT GGC TGG CTA GG

Chemokine (C-C motif) ligand 8

CCL8

S67954

667

TGA GAC CAA CTC AGC AAT CG

AGT GGA AGG ACC TCA CCA TC

Colony stimulating factor 2

CSF2

U22385

602

GAG GAT GTG GCT GCA GAA CC

CCT GTA TCA GGG TCA ACA TGG

Colony stimulating factor 2 receptor α

CSF2RA

BE480577

455

CCA AAT GCC AGC CTA AAT GTG

CGA GAG GTC TTG GAG GTG AC

Complement component 5 receptor 1

C5R1

X65861

396

ATC ATC CAG CAC AAC CAC TG

GCA GGA GGA AGG TGT AAC AG

FcγRIα (CD64)

FCGR1A

AF162866

630

TCT GGT CAA CCT GAG CTG TG

TAA GAT GCC AAG GGA GTT GC

FcγRIIIb (CD16b)

FCGR3B

AF132036

502

CTT TCA GCT GAC ACG CAA AC

TTT CCT GCT GCT CCC AGA G

Inhibitor of kappa light polypeptide gene enhancer in B-cells, kinase β

IKBKB

AJ414556

688

TTT GAC AAC AGC AGG GTC AC

TGC AGA CCA CGG TCT TAC TG

Inhibitor of kappa light polypeptide gene enhancer in B-cells, kinase γ

IKBKG

AJ414557

694

ATG GAG TGC ATC GAG TAG GG

AGA GAG GAG CTC ACC TTG TG

Insulin-like growth factor 1

IGF1

X15726

661

GTG ATC TGA GGA GGC TGG AG

AGG TGA TGG AGA AGG GAG TG

Integrin αV (CD51)

ITGAV

BM431105

505

CAT ATC TGC GGG ATG AAT CTG

TCC ATC TCT GAT TGC TGG TG

Integrin α6 (CD49f)

ITGA6

CK970520

554

TAC CCT GAT GTT GCT GTT GG

ATG TTG TCG TCT CCA CAT CC

Integrin β1 (CD29)

ITGB1

AF468058

691

TGT GTT TCA CTT CGC TGG AG

TTC GCT GTT CAC CTC ATC TG

Integrin β3 (CD61)

ITGB3

CN791459

530

TGT TCT CCA GCT CAT TGT GG

TCT TGC CAA AGT CAC TGC TG

Intercellular adhesion molecule (CD50)

ICAM3

L41844

628

CCA AGA GAT TGT CTG CAA CG

GCC CAA GAT CGC TAA CAC TC

Interferon α

IFNA

Z46508

439

GGT CCT GAT GCT CCT GAG AC

CCT GCA AGT TTG TTG AGG AAG

Interferon β1

IFNB1

M15477

568

TCT CCA CCA CAG CTC TTT CC

TCT CAC CTC AAA GTC ACA TTG

Interferon γ

IFNG

M39867

561

GGA GCT ACC GAT TTC AAC TAC TCC G

GCA GGC AGG AGG ACC ATT ACG

Interferon regulatory factor 2

IRF2

AJ490936

686

AAA CTG GGC CAT CCA TAC AG

TGT TGG ACG TGA CAA AGG TG

Interleukin 1β

IL1B

M37211

699

AAG GCT CTC CAC CTC CTC TC

TGG TTG CTC ATC AGA AGC TG

Interleukin 1 receptor-associated kinase 1

IRAK1

BG692698

405

CTC TCT CCT GGC CTC AGC

CTT CCG CCT CCT CTT CAA C

Interleukin 2

IL2

M13204

498

GGT CGT TCA TGT CAG CAA TG

GAG GCA CTT AGT GAT CAA GTC

Interleukin 4

IL4

M77120

451

GCA TTG TTA GCG TCT CCT GGT AAA C

CTT CAT AAT CGT CTT TAG CCT TTC C

Interleukin 6

IL6

X57317

620

ATG AAC TCC CGC TTC ACA AGC

TAC TTC ATC CGA ATA GCT CTC

Interleukin 8

IL8

AF232704

660

CAG CAG AGC TCA CAA GCA TC

AAA AGC AGG ATT TCC AGA TCG

Interleukin 12A

IL12A

U14416

350

CAG CAA CAC GCT ACA GAA GG

CCT GCA TCA GCT CAG CAA TA

Interleukin 12B

IL12B

U11815

553

CCT GTC ACA AAG GAG GCG AGG C

CGT CAG GGA GAA GTA GGA ATG CGG

Interleukin 13

IL13

CB454692

477

CCT CAA TTT CTT CCT GTG CTG

ATG GTC AGG ATC TGC CTC TG

Interleukin 15

IL15

U42433

323

ATC CAG TGC TAC TTG TGT TTA C

GCT GTT TGC TAG CAT GGT AAG

MHC class II DYA

BOLA-DYA

AJ580583

623

ACC ACT TGG AAC AGC CAC TC

CAG TGC TCC ACT CTG CAG TC

Myeloid differentiation primary response gene

MYD88

AJ634627

431

GTG GTG GTG GTC TCT GAC G

GTG CCA CGT GTT CTA TGC AG

Nuclear factor of kappa light polypeptide gene enhancer in B-cells 2 (p49/p100)

NFKB2

CO259349

411

GGC TAT ACC CAG TCC ACC TG

TGA GGT CAA GAG GTG TGT GG

Prostaglandin-endoperoxide synthase 2

PTGS2

AF031698

651

CCA GAG CTC TTC CTC CTG TG

GCA GCT CTG GGT CAA ACT TC

Selectin P ligand (CD162)

SELPLG

BF654668

421

ACC ATC TTC CTC GTG TGC AC

CTA CCG GAG GTT CTG TTT GC

SH3-domain kinase binding protein 1

SH3KBP1

AJ819523

340

GTC AGG GCT TGA AAG GGA AG

GCC TTC TGT TCC TGC CAT AC

Signal transducer & activator or transcription 1

STAT1

BE588937

519

GCG AGT CAG ATC CAA GAT AG

ATC CTG AAG GTT ACG CTT GC

Tissue inhibitor of metalloproteinase 2

TIMP2

M32303

617

GGG CCA AAG CAG TCA ATA AG

TTC ATG CTG TGT CCA GGA AG

Toll-like receptor 7

TLR7

AY487802

693

GCC TTC TGA TTT CCA GCA TC

TTT GTC AGG CAC CTG ATT TG

Toll-like receptor 8

TLR8

AY642125

362

GGT GAT GAA TGA GCT GCG C

CTT TGC CAA AAC AAG CCC TC

Toll-like receptor 9

TLR9

AJ509825

694

TCA CCA GCC TCT CCT TAA TCT C

GAG AGA ACT GTC CTT CAA CAC C

Transforming growth factor β1

TGFB1

M36271

668

GCC ATA CTG GCC CTT TAC AA

GCT GAC GAA CAC AGC AGT TC

Transforming growth factor β3

TGFB3

CA034974

374

AGG TGT GGA AAT CAG CAT CC

CTG AGC AGA AGT TGG CAT AG

Transforming growth factor β receptor II

TGFBR2

CK772005

701

GTG TTG AGG GAG CCA TCT TC

AAC CTG CAG GAG TAC CTG AC

Tumour necrosis factor α

TNF

AF011926

660

AGC TGG CGG AGG AGG TGC TC

CAG GGC GAT GAT CCC AAA GTA GAC C

TNFRSF6 (fas)

FAS

U34794

645

TTC GGA AGA ACG GTA TGG AG

CCT GTG GAT AGG CAT GTG TG

  1. List of the genes chosen for amplicon construction. The sequences used to design the primers and the sizes of the clone inserts are listed.