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Table 2 Amplicons generated to represent 60 immunologically important genes.

From: Development and validation of a bovine macrophage specific cDNA microarray

Gene Symbol Accession No. Size (bp) Forward Primer 5'-3' sequence Reverse Primer 5'-3' sequence
Annexin-A5 ANXA5 CO883855 700 ATG GCA CAG GTT CTC AGA GG CCA AAT TGC CAG AAG TCT CC
Baculoviral IAP-repeat containing 5 BIRC5 BE486047 451 CCT GGC AGC TCT ACC TCA AG CCC TGG AAA CGA CGT GTC TG
B-cell CLL/lymphoma 3 BCL3 CK771977 537 CTT CTT GGC ACT CGG TGT TC GAC CAG AGG CAA TTT ACT ACC
Capping protein (actin filament) gelsolin like CAPG AY219899 615 TAT GGA GGG TGG AGA AGC TG TGG GAC CCA AGA CCT GTA TC
Casein kinase II α1 CSNK2A1 X54962 405 GTT AAT ACA CAC AGA CCC CGA GCT ATG GCA GTA ATC AAG GGC C
Caspase 3 CASP3 CK958902 679 GTG GAT GCA GCA AAC CTC AG CCA TTT GCT CAC TTG GCA TAC
Caspase 7 CASP7 CK436554 549 TCT TCC AAA GTC ACC ACC AG GGA GTG GCT CTT GGT TTG TG
Caspase recruitment domain family member 4 CARD4 CO886987 630 ATG TCG GAG CCA GGT ACA TC GAA GTC CCA CCT CAG CAG AC
CD2 antigen CD2 CN789613 544 CAG AAC TGT GAG CTG CAA GG TTG CTG GTG AAC TTG TGT GC
CD3 antigen γ CD3G CD445323 518 GAA GGA GTT CGC CAG TCA AG TGA TGA GAA TAG CCA ATA AGC AC
CD40 ligand CD40LG Z48469 652 TAA CGC AGC ATG ATC GAA AC AGA ACT GTG GGT GTT TGC AG
CD63 antigen CD63 AJ012589 606 TTC TCC TGC TGG TCT TCT GC GGA TCT CCA CAA AGG CAA TG
CD80 antigen CD80 BF039039 522 GGT ACT CCA ACC AGC GTC TC AGG TGT AGG TGC CAC TGT CC
CD86 antigen CD86 AJ291475 674 GCC TTC TTC AAC GAG ACT GG GCA ATC CAG AGG ATG TGG TC
CD200 receptor 1 CD200R1 CK831048 552 CTG TGG ACA GAA AGC AGA GC GGT TCT GAC ACC TTT ATC CAG
Chemokine (C motif) ligand 1 XCL1 AF354057 411 TCA GCC TCT TAC TGC ACA GC AAA TGA GGT GGC TGG CTA GG
Chemokine (C-C motif) ligand 8 CCL8 S67954 667 TGA GAC CAA CTC AGC AAT CG AGT GGA AGG ACC TCA CCA TC
Colony stimulating factor 2 CSF2 U22385 602 GAG GAT GTG GCT GCA GAA CC CCT GTA TCA GGG TCA ACA TGG
Colony stimulating factor 2 receptor α CSF2RA BE480577 455 CCA AAT GCC AGC CTA AAT GTG CGA GAG GTC TTG GAG GTG AC
Complement component 5 receptor 1 C5R1 X65861 396 ATC ATC CAG CAC AAC CAC TG GCA GGA GGA AGG TGT AAC AG
FcγRIα (CD64) FCGR1A AF162866 630 TCT GGT CAA CCT GAG CTG TG TAA GAT GCC AAG GGA GTT GC
FcγRIIIb (CD16b) FCGR3B AF132036 502 CTT TCA GCT GAC ACG CAA AC TTT CCT GCT GCT CCC AGA G
Inhibitor of kappa light polypeptide gene enhancer in B-cells, kinase β IKBKB AJ414556 688 TTT GAC AAC AGC AGG GTC AC TGC AGA CCA CGG TCT TAC TG
Inhibitor of kappa light polypeptide gene enhancer in B-cells, kinase γ IKBKG AJ414557 694 ATG GAG TGC ATC GAG TAG GG AGA GAG GAG CTC ACC TTG TG
Insulin-like growth factor 1 IGF1 X15726 661 GTG ATC TGA GGA GGC TGG AG AGG TGA TGG AGA AGG GAG TG
Integrin αV (CD51) ITGAV BM431105 505 CAT ATC TGC GGG ATG AAT CTG TCC ATC TCT GAT TGC TGG TG
Integrin α6 (CD49f) ITGA6 CK970520 554 TAC CCT GAT GTT GCT GTT GG ATG TTG TCG TCT CCA CAT CC
Integrin β1 (CD29) ITGB1 AF468058 691 TGT GTT TCA CTT CGC TGG AG TTC GCT GTT CAC CTC ATC TG
Integrin β3 (CD61) ITGB3 CN791459 530 TGT TCT CCA GCT CAT TGT GG TCT TGC CAA AGT CAC TGC TG
Intercellular adhesion molecule (CD50) ICAM3 L41844 628 CCA AGA GAT TGT CTG CAA CG GCC CAA GAT CGC TAA CAC TC
Interferon α IFNA Z46508 439 GGT CCT GAT GCT CCT GAG AC CCT GCA AGT TTG TTG AGG AAG
Interferon β1 IFNB1 M15477 568 TCT CCA CCA CAG CTC TTT CC TCT CAC CTC AAA GTC ACA TTG
Interferon γ IFNG M39867 561 GGA GCT ACC GAT TTC AAC TAC TCC G GCA GGC AGG AGG ACC ATT ACG
Interferon regulatory factor 2 IRF2 AJ490936 686 AAA CTG GGC CAT CCA TAC AG TGT TGG ACG TGA CAA AGG TG
Interleukin 1β IL1B M37211 699 AAG GCT CTC CAC CTC CTC TC TGG TTG CTC ATC AGA AGC TG
Interleukin 1 receptor-associated kinase 1 IRAK1 BG692698 405 CTC TCT CCT GGC CTC AGC CTT CCG CCT CCT CTT CAA C
Interleukin 2 IL2 M13204 498 GGT CGT TCA TGT CAG CAA TG GAG GCA CTT AGT GAT CAA GTC
Interleukin 4 IL4 M77120 451 GCA TTG TTA GCG TCT CCT GGT AAA C CTT CAT AAT CGT CTT TAG CCT TTC C
Interleukin 6 IL6 X57317 620 ATG AAC TCC CGC TTC ACA AGC TAC TTC ATC CGA ATA GCT CTC
Interleukin 8 IL8 AF232704 660 CAG CAG AGC TCA CAA GCA TC AAA AGC AGG ATT TCC AGA TCG
Interleukin 12A IL12A U14416 350 CAG CAA CAC GCT ACA GAA GG CCT GCA TCA GCT CAG CAA TA
Interleukin 12B IL12B U11815 553 CCT GTC ACA AAG GAG GCG AGG C CGT CAG GGA GAA GTA GGA ATG CGG
Interleukin 13 IL13 CB454692 477 CCT CAA TTT CTT CCT GTG CTG ATG GTC AGG ATC TGC CTC TG
Interleukin 15 IL15 U42433 323 ATC CAG TGC TAC TTG TGT TTA C GCT GTT TGC TAG CAT GGT AAG
MHC class II DYA BOLA-DYA AJ580583 623 ACC ACT TGG AAC AGC CAC TC CAG TGC TCC ACT CTG CAG TC
Myeloid differentiation primary response gene MYD88 AJ634627 431 GTG GTG GTG GTC TCT GAC G GTG CCA CGT GTT CTA TGC AG
Nuclear factor of kappa light polypeptide gene enhancer in B-cells 2 (p49/p100) NFKB2 CO259349 411 GGC TAT ACC CAG TCC ACC TG TGA GGT CAA GAG GTG TGT GG
Prostaglandin-endoperoxide synthase 2 PTGS2 AF031698 651 CCA GAG CTC TTC CTC CTG TG GCA GCT CTG GGT CAA ACT TC
Selectin P ligand (CD162) SELPLG BF654668 421 ACC ATC TTC CTC GTG TGC AC CTA CCG GAG GTT CTG TTT GC
SH3-domain kinase binding protein 1 SH3KBP1 AJ819523 340 GTC AGG GCT TGA AAG GGA AG GCC TTC TGT TCC TGC CAT AC
Signal transducer & activator or transcription 1 STAT1 BE588937 519 GCG AGT CAG ATC CAA GAT AG ATC CTG AAG GTT ACG CTT GC
Tissue inhibitor of metalloproteinase 2 TIMP2 M32303 617 GGG CCA AAG CAG TCA ATA AG TTC ATG CTG TGT CCA GGA AG
Toll-like receptor 7 TLR7 AY487802 693 GCC TTC TGA TTT CCA GCA TC TTT GTC AGG CAC CTG ATT TG
Toll-like receptor 8 TLR8 AY642125 362 GGT GAT GAA TGA GCT GCG C CTT TGC CAA AAC AAG CCC TC
Toll-like receptor 9 TLR9 AJ509825 694 TCA CCA GCC TCT CCT TAA TCT C GAG AGA ACT GTC CTT CAA CAC C
Transforming growth factor β1 TGFB1 M36271 668 GCC ATA CTG GCC CTT TAC AA GCT GAC GAA CAC AGC AGT TC
Transforming growth factor β3 TGFB3 CA034974 374 AGG TGT GGA AAT CAG CAT CC CTG AGC AGA AGT TGG CAT AG
Transforming growth factor β receptor II TGFBR2 CK772005 701 GTG TTG AGG GAG CCA TCT TC AAC CTG CAG GAG TAC CTG AC
Tumour necrosis factor α TNF AF011926 660 AGC TGG CGG AGG AGG TGC TC CAG GGC GAT GAT CCC AAA GTA GAC C
TNFRSF6 (fas) FAS U34794 645 TTC GGA AGA ACG GTA TGG AG CCT GTG GAT AGG CAT GTG TG
  1. List of the genes chosen for amplicon construction. The sequences used to design the primers and the sizes of the clone inserts are listed.