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Table 3 Factors affecting intensity measurements.

From: Development and validation of a bovine macrophage specific cDNA microarray

Effect

Variance

SE (variance)

Variance %

Variance 0 hours

Variance 2 hours

Variance 16 hours

Variance 0 hours (%)

Variance 2 hours (%)

Variance 16 hours (%)

Slide

0.032

0.015

2

0.035

0.161

0.054

2

10

4

Gene

1.146

0.006

79

1.126

1.152

1.160

80

71

77

Replicate Spots

0.135

0.001

9

0.174

0.123

0.107

12

8

7

Channel

0.145

0.001

10

0.069

0.179

0.186

5

11

12

  1. The contributions to variation in log(intensity) of slide, gene, replicate spots and channel, together with their standard errors and percentage variance accounted for. Equivalent estimates and percentages are presented separately for the 3 time points