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Table 8 Details of the qRT-PCR primers and amplicons.

From: Development and validation of a bovine macrophage specific cDNA microarray

Gene Gene Symbol Clone Direction Sequence (5'-3') Size (bp)
CD9 antigen CD9 BE751736 F TTG GAC TAT GGC TCC GAT TC 261
    R CCT TGA TCA CCT CCT CCT TG  
CD44 antigen CD44 AJ816969 F TAC CCA CCC ACA AGA GAA CC 102
    R AAC CCA TTA CCA GTC ATC CTT G  
CD86 antigen CD86 pCD86 F GAA GGT CCC AAG GAT TGG TT 136
    R GAT GAG CAG GTC AAA TTG ATG A  
Chemokine (C-C motif) ligand 2 CCL2 AJ821161 F TCG CCT GCT GCT ATA CAT TC 274
    R AGA GGG CAG TTA GGG AAA GC  
Chemokine (C-C motif) receptor 1 CCR1 BE756795 F AAA TGA GAA GAA GGC CAA AGC 140
    R TGC TCT GCT CAC ACT TAC GG  
Complement component 1, r subcomponent C1R AJ818500 F AGA TTT GTC CGT CTG CCA GT 183
    R CCC AGC GGT CAT TTC TCT TA  
Fibronectin 1 FN1 AJ819936 F TAT CTT GGT GCC ATT TGC TC 258
    R ATC CAC GCT TGT TTC TCT GG  
Interleukin 6 IL6 pIL6 F TGA GTG TGA AAG CAG CAA GG 289
    R CAG CAG GTC AGT GTT TGT GG  
Prion protein PRNP AJ821011 F TGT GTA TGG TGG GAT TGG AA 197
    R GCT GGG TCA TCA AAG AAA TG  
V-fos FBJ murine osteosarcoma viral oncogene FOS BE755117 F CTT CTC CAG CAT GGG TTC TC 257
    R TCT GAG CTC TGC CTC CTG TC  
Chromosome 13 open reading frame 8 C13orf8 AJ817183 F AGC AGT GAC CAA GAG CAG GT 205
    R TCA TAG CAC GAC AGC AAC AA  
  1. F and R denote forward and reverse primers respectively.