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Table 1 Known unigenes showing significant expression differences between symbiotic and aposymbiotic anemones. Identification was based on significant blast hits (E values < 10-4) from GenBank. Ratios of expression from all microarray analyses and selected Q-RT-PCR analyses are shown. Arrows indicate the direction of expression as a function of symbiosis: up- or down-regulation. Those genes highlighted in bold are discussed in the text. Significance is indicated as follows: P < 0.05 (*), P < 0.01 (**).

From: Transcriptome analysis of a cnidarian – dinoflagellate mutualism reveals complex modulation of host gene expression

Unigenes grouped based on functional category

Genbank Accession ID

OSU-CGRB ID

Expression as function of symbiosis

   

Microarray

Q-RT-PCR

Cell Growth and Maintenance

von Willebrand factor

DQ309533

AeOSU006130

↑ 1.92 *

↑ 1.82 *

Tubulin alpha chain

DQ309523

AeOSU007825

↓ 1.20 *

 

Myosin regulatory light chain 2

DQ309534

AeOSU008456

↓ 1.37 *

 

Beta-tubulin

DQ309552

AeOSU009795

↓ 1.39 *

 

Collagen alpha-1 chain, type XII

DQ309546

AeOSU000073

↓ 1.94 **

 

Metabolism and Energy Pathways

Phytanoyl-CoA hydroxylase

DQ309528

AeOSU005830

↑ 1.54 *

↑1.59 *

Selenoprotein W1

DQ309535

AeOSU002895

↑ 1.42 *

 

Long chain acyl-CoA thioesterase

DQ309538–DQ309539

AeOSU003383

↑ 1.28 *

 

ATP synthase, H+ transporting, mitochondrial F0 complex

DQ309536

AeOSU000538

↓ 1.29 *

 

Medium chain S-acyl fatty acid synthetase thio ester hydrolase

DQ309537

AeOSU000658

↓ 1.56 *

↓ 1.32 *

Copper/zinc superoxide dismutase (SOD)

DQ309550

AeOSU001001

↓ 1.58 *

↓ 1.89 *

Protein metabolism

60S ribosomal protein L26

DQ309540

AeOSU004867

↑ 3.43 **

 

Plasma glutamate carboxypeptidase

DQ309521

AeOSU001186

↑ 1.70 *

 

Nucleic Acid Metabolism

Activating transcription factor 4 (ATF4)

DQ309526

AeOSU001247

↑ 1.51 *

↑ 2.49 *

Histone H3.3

DQ309522

AeOSU007640

↑ 1.22 *

 

DEAD box polypeptide 48

DQ309551

AeOSU006260

↓ 1.30 *

 

Signal Transduction &cell communication

Oncoprotein-induced transcript 3

DQ309527

AeOSU009888

↑ 1.40 *

 

Scavenger receptor class B, type 1 (CD36)

DQ309525

AeOSU008133

↑ 1.34 *

 

Sphingosine 1-phosphate phosphatase 2

DQ309531–DQ309532

AeOSU005519

↓ 1.50 *

↓ 1.63 *

Betaine homocysteine methyl transferase

DQ309529

AeOSU002910

↓ 1.56 *

↓ 4.18 *

Calcium/calmodulin-dependent protein kinase IV

DQ309544

AeOSU005891

↓ 1.57 *

 

Prohibitin protein

DQ309542–DQ309543

AeOSU001676

↓ 2.21 *

 

Transport

Membrane-bound transferrin-like protein p97

DQ309541

AeOSU001619

↓ 1.29 *

 

Unclassified

Death-associated protein 1 (DAP-1)

DQ309530

AeOSU008955

↑ 1.39 *

 

Human headcase homolog (HECA)

DQ309548

AeOSU009119

↑ 1.33 *

 

Guanine nucleotide binding protein, gamma 5 or 12

DQ309547

AeOSU008880

↑ 1.29 *

 

SEC31-like 2 isoform A

DQ309524

AeOSU009211

↑ 1.17 *

 

Methylmalonate semialdehyde dehydrogenase

DQ309545

AeOSU005938

↓ 1.37 *