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Table 4 Primer sequences and PCR conditions

From: A biological question and a balanced (orthogonal) design: the ingredients to efficiently analyze two-color microarrays with Confirmatory Factor Analysis

Gene Primer sequences T a (C°) Cycles
GAPDH F: 5'-caccaccatggagaaggctgg-3' R: 5'-ccaaagttgtcatggatgacc-3' 65 30
COL3A1 F: 5'-agcctccaactgctccta-3' R: 5'-gtccgggtctacctgatt-3' 56 30
ENO2 F: 5'-gtctgctgctcaaggtcaac-3' R: 5'-tccaggcaagcagaggaatc-3' 54 30
FGF18 F: 5'-cttcctgctgctgtgcttcc-3' R: 5'-cactccttgctggtgccatc-3' 57 30
JUN F: 5'-gctatctaggtggagttg-3' R: 5'-gcacatgccacttgatac-3' 46 30
LHX2 F: 5'-tgaaggacagcctggtctac-3' R: 5'-gagctgcttcaagtccttgg-3' 56 30
MEIS1 F: 5'-gctgttccagcatctaacac-3' R: 5'-tgttgctgaccgtccattac-3' 50 30
MEIS2 F: 5'-gatcacgccgttatgttgcc-3' R: 5'-gctggagttcgagtgatgag-3' 49 30
PBX3 F: 5'-caggaagcaggacatcgg-3' R: 5'-ttggctctgtaatctgagtgtt-3' 56 30
PDGFRL F: 5'-gtggagctaccctgcgtatc-3' R: 5'-ctgggagaaggtacaaagagttc-3' 60 35
PRICKLE1 F: 5'-aggtacggtattgccagtctt-3' R: 5'-cgaacactgcaacttcacctc-3' 60 35
SAT F: 5'-tcactcgccgaggttccttg-3' R: 5'-acagcagcactcctcactcc-3' 53 30
SHB F: 5'-gtttaatggcaacgagaagcg-3' R: 5'-tcctcacagccacgggatag-3' 60 35
TIMP2 F: 5'-ggaaacgacatttatggcaacc-3' R: 5'-acccagtccatccagaggc-3' 60 30
TLX1 F: 5'-acctcactggcctcaccttc-3' R: 5'-cagaccacggctgcagattc-3' 57 30
TOP1 F: 5'-gaaggaacagctagcagatg-3' R: 5'-agaactctgcctcttgagac-3' 50 30
UACA F: 5'-cactgaatgacacgttagcca-3' R: 5'-atcctgcacctttctcatgct-3' 60 35
  1. Ta, annealing temperature.