Table 4 Primer sequences and PCR conditions
Gene | Primer sequences | T a (C°) | Cycles |
|---|---|---|---|
GAPDH | F: 5'-caccaccatggagaaggctgg-3' R: 5'-ccaaagttgtcatggatgacc-3' | 65 | 30 |
COL3A1 | F: 5'-agcctccaactgctccta-3' R: 5'-gtccgggtctacctgatt-3' | 56 | 30 |
ENO2 | F: 5'-gtctgctgctcaaggtcaac-3' R: 5'-tccaggcaagcagaggaatc-3' | 54 | 30 |
FGF18 | F: 5'-cttcctgctgctgtgcttcc-3' R: 5'-cactccttgctggtgccatc-3' | 57 | 30 |
JUN | F: 5'-gctatctaggtggagttg-3' R: 5'-gcacatgccacttgatac-3' | 46 | 30 |
LHX2 | F: 5'-tgaaggacagcctggtctac-3' R: 5'-gagctgcttcaagtccttgg-3' | 56 | 30 |
MEIS1 | F: 5'-gctgttccagcatctaacac-3' R: 5'-tgttgctgaccgtccattac-3' | 50 | 30 |
MEIS2 | F: 5'-gatcacgccgttatgttgcc-3' R: 5'-gctggagttcgagtgatgag-3' | 49 | 30 |
PBX3 | F: 5'-caggaagcaggacatcgg-3' R: 5'-ttggctctgtaatctgagtgtt-3' | 56 | 30 |
PDGFRL | F: 5'-gtggagctaccctgcgtatc-3' R: 5'-ctgggagaaggtacaaagagttc-3' | 60 | 35 |
PRICKLE1 | F: 5'-aggtacggtattgccagtctt-3' R: 5'-cgaacactgcaacttcacctc-3' | 60 | 35 |
SAT | F: 5'-tcactcgccgaggttccttg-3' R: 5'-acagcagcactcctcactcc-3' | 53 | 30 |
SHB | F: 5'-gtttaatggcaacgagaagcg-3' R: 5'-tcctcacagccacgggatag-3' | 60 | 35 |
TIMP2 | F: 5'-ggaaacgacatttatggcaacc-3' R: 5'-acccagtccatccagaggc-3' | 60 | 30 |
TLX1 | F: 5'-acctcactggcctcaccttc-3' R: 5'-cagaccacggctgcagattc-3' | 57 | 30 |
TOP1 | F: 5'-gaaggaacagctagcagatg-3' R: 5'-agaactctgcctcttgagac-3' | 50 | 30 |
UACA | F: 5'-cactgaatgacacgttagcca-3' R: 5'-atcctgcacctttctcatgct-3' | 60 | 35 |