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Table 3 Comparison of gene expression levels between microarray and real-time RT-PCR

From: Butyrate induces profound changes in gene expression related to multiple signal pathways in bovine kidney epithelial cells

Accession

Annotation

Microarray*

Real-time PCR**

TC281450

BUB1 budding uninhibited by benzimidazoles 1 homolog beta (BUB1B)

0.1

0.1

TC266701

Fas apoptotic inhibitory molecule (FAIM)

0.1

0.3

TC277820

MAD2 mitotic arrest deficient-like 1 variant (MAD2L1)

0.1

0.1

TC261165

Centromere protein A (CENPA)

0.1

0.2

TC289876

Proliferating cell nuclear antigen (PCNA)

0.1

0.1

TC290192

Spindle pole body component 24 (SPBC24)

0.1

0.2

TC269605

Hepatocyte growth factor (scatter factor, HGF)

0.1

0.4

TC267353

Kinesin family member 20A (KIF20A)

0.1

0.1

TC260258

Proteasome subunit, beta type 8 (PSMB8)

0.1

0.2

TC262855

Cyclin-dependent kinase 4 (CDK4)

0.1

0.6

TC290639

Minichromosome maintenance protein 3 associated protein (MCM3AP)

0.2

0.5

TC289874

Insulin-like growth factor-binding protein 6 (IGFBP6)

0.2

0.3

TC262471

Heat shock 90kD protein 1, alpha (HSP90AA1)

0.2

0.5

TC295998

Activator of S phase kinase

0.3

0.3

TC277755

CSE1 chromosome segregation 1-like (CSE1L)

0.3

0.4

TC261112

Profilin 1 (PFN1)

0.4

0.4

TC294142

β-catenin (CTNNB1)

1.4

5.8

TC260308

GM2 ganglioside activator protein (GM2A)

2.3

6.6

TC267034

similar to LIM domain containing 2 (LIMD2)

2.3

4.2

TC292975

unknown

2.5

17.1

TC276824

Dual specificity protein phosphatase 1 (MKP-1)

2.8

6.6

TC260543

Cathepsin D (CTSD)

3.0

5.1

TC277986

Cathepsin L precursor (CTSL)

3.2

2.7

TC276574

Protease, serine, 11 [IGF binding] (PRSS11)

3.8

11.2

TC298909

Serine/threonine kinase 32C (STK32C)

4.3

26.2

TC265800

Activating transcription factor 3 (ATF3)

4.5

10.2

TC277745

CITED2

6.3

47.6

TC276741

Insulin-like growth factor 2 (IGF2)

6.5

100.0

TC280815

Matrix metalloproteinase 13 (MMP13)

8.3

87.8

TC265993

Inhibin, beta A (INHBA)

9.5

82.8

  1. * fold change (microarray) = mean background-adjusted, normalized signal intensity from the butyrate treated group divided by mean background-adjusted, normalized signal intensity from the control group.
  2. ** fold change (real-time PCR): expression in the control group was set at 1.0. The values below 1.0 indicated down-regulation by butyrate.