Figure 2

Monitoring of disturbances in gene expression stability in chemostat cultures. A. Dry weight (blue squares), off gas CO₂ concentration (black line), protein concentration (g g^-1 DW) (red diamonds) and cellobiohydrolase activity (nkat g^-1 DW) (orange circles) and ammonium concentration (green triangles) in chemostat cultures IV–VI (D = 0.03 h^-1). B. Average fold-change of expression from predicted steady state of 30 marker genes (F _SS ). Error bars indicate the average deviation in Fss between the different marker genes. C. Marker genes show up (green circles) or down (red squares)-regulation during the continuous phase of the cultures. Expression levels as log₂ expression ratios from the F _ma predicted steady state expression level