Unrooted tree of L. major SNARE domain-containing proteins. A 71 amino acids-long SNARE motif (see Figure S1 from Additional file 1) from L. major predicted SNARE proteins was aligned using the Clustal W algorithm and a phylogenetic tree was generated as described in the Methods section. Selected characterised members from the Qa, Qb, Qc and R groups were included in the analysis (boxed). They are, respectively, human Syntaxin 5A (NP_012262), yeast Vti1p (Q04338), yeast Bet1 (NP_012262) and human Vamp1 (CAA88760). Branches with bootstrap values > 45 in green and > 90 in red. The scale bar represents 0.2 mutational changes per residue (20 PAM units). L. major sequences are labelled with their identifier in the GeneDB database .