Figure 4

GFP-LmjF35.2720 and GFP-LmjF33.1340 label punctate structures. Localisation of GFP-fused LmjF33.1340 (A) and LmjF35.2720 (B) in live (left hand side) or fixed (right hand side) L. major promastigotes. Corresponding DIC images are shown; the GFP fluorescence signal is shown in green and immuno-labelling with Golgi marker Rab1 is shown in red. The merged fluorescent signals, together with the DAPI staining of DNA (blue; n: nucleus; k: kinetoplast) are shown in the enlarged image. Arrowheads point the predominantly fluorescent signal located close to the Golgi staining. Scale bar = 10 μm.