GFP-LmjF32.0070 is associated with the Golgi. (A) Localisation of GFP-fused LmjF32.0070 in live L. major promastigotes (insets display the corresponding DIC images). Arrowheads point the GFP-labelled structure(s) in a single promastigote (left), or in a dividing cell before (middle) or during (right) cytokinesis. (B) Co-localisation of GFP-LmjF32.0070 (green) and immuno-labelled Golgi protein Rab1 (red) in fixed L. major promastigotes. The merged signals, together with the DAPI staining of DNA (blue; n: nucleus; k: kinetoplast) are shown in the enlarged image. Co-localised signal is shown in yellow. Scale bar = 10 μm.