Table 2 Primers used to amplify L. major Qa SNAREs.
Primer name | Primer sequence | Gene amplified | Fragment size (bp) |
|---|---|---|---|
OL1970 | CGTAGATCT ATGCAGGAAATGGGGG | 5' of LmjF29.0070 | |
OL1971 | CTTGGTACC TCAGTTCATGAGCAGA | 3' of LmjF29.0070 | 755 |
OL2150 | AAGAGATCT ATGGATCGTCTTCCAC | 5' of LmjF28.1470 | |
OL2038 | AAGAGATCT ATGGCCGAGCTGCAGC | 5' of short LmjF28.1470/LmjF28.1480 | |
OL2039 | GTCGATATC CTACAGAACCGTAAAC | 3' of LmjF28.1470 | 864/1107 |
OL2048 | ACGGATATC TCAGTCCTCCCAGGCC | 3' of LmjF28.1480 | 801 |
OL2040 | AAGAGATCT ATGCCCTTAGAGAATG | 5' of LmjF19.0120 | |
OL2041 | ACGGATATC TCATCGGGTGAGAACC | 3' of LmjF19.0120 | 690 |
OL2042 | AAGAGATCT ATGCGTTTGACGCAGC | 5' of LmjF32.0070 | |
OL2043 | ACGGATATCT TAGCGCACCACGAGG | 3' of LmjF32.0070 | 738 |
OL2044 | AAGAGATCT ATGGAACTGAAAGTCA | 5' of LmjF33.1340 | |
OL2045 | ACGCTCGAG TCACATAAGAATGCAG | 3' of LmjF33.1340 | 828 |
OL2046 | ACGGGTACC ATGGCGACTCGCGACC | 5' of LmjF35.2720 | |
OL2047 | ACGGATATC TCAGGTAATGGCCTTG | 3' of LmjF35.2720 | 909 |