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Table 2 Primers used to amplify L. major Qa SNAREs.

From: The SNARE protein family of Leishmania major

Primer name Primer sequence Gene amplified Fragment size (bp)
OL1970 CGTAGATCT ATGCAGGAAATGGGGG 5' of LmjF29.0070  
OL1971 CTTGGTACC TCAGTTCATGAGCAGA 3' of LmjF29.0070 755
OL2150 AAGAGATCT ATGGATCGTCTTCCAC 5' of LmjF28.1470  
OL2038 AAGAGATCT ATGGCCGAGCTGCAGC 5' of short LmjF28.1470/LmjF28.1480  
OL2039 GTCGATATC CTACAGAACCGTAAAC 3' of LmjF28.1470 864/1107
OL2048 ACGGATATC TCAGTCCTCCCAGGCC 3' of LmjF28.1480 801
OL2040 AAGAGATCT ATGCCCTTAGAGAATG 5' of LmjF19.0120  
OL2041 ACGGATATC TCATCGGGTGAGAACC 3' of LmjF19.0120 690
OL2042 AAGAGATCT ATGCGTTTGACGCAGC 5' of LmjF32.0070  
OL2043 ACGGATATCT TAGCGCACCACGAGG 3' of LmjF32.0070 738
OL2044 AAGAGATCT ATGGAACTGAAAGTCA 5' of LmjF33.1340  
OL2045 ACGCTCGAG TCACATAAGAATGCAG 3' of LmjF33.1340 828
OL2046 ACGGGTACC ATGGCGACTCGCGACC 5' of LmjF35.2720  
OL2047 ACGGATATC TCAGGTAATGGCCTTG 3' of LmjF35.2720 909
  1. Specific primers used to amplify the specified L. major genes to give products of which the sizes are indicated on the right. Restriction sites used for cloning are in bold.