Figure 5
Structural models showing conservation of allergen and epitope structure between enolase allergens and allergen homologues. Conserved non-epitope structure is shown in blue. Epitope structure that is identical in all allergens studied and also identical in allergen-like proteins is shown in red. Epitope amino acids not conserved between experimentally determined cross-reacting allergen epitopes are shown in green and amino acids differing from epitope in allergen-like proteins are shown in yellow. A: View of enolase Cla h 6 predicted structure as a space fill model showing conserved epitope present in all fungi. B: Reverse view of Cla h 6 showing epitopes conserved in ascomycetes C: Reverse view of Cla h 6 showing epitopes conserved in yeasts, C. albicans, C. tropicalis, S. cerevisiae and R, mucilaginosa. D: Reverse view of Cla h 6 showing epitopes conserved in basidiomycetes C. cinereus C. neoformans and U. maydis E: Representative enolase structures consisting of Cla h 6, Sac c enolase and C. cinereus enolases superimposed. F: Enolase epitope structure for ascomycetes. The exposed epitope structure is displayed as ribbon and the buried portion of the epitope is shown as a blue CA trace. G: Enolase epitope structure for yeasts. The exposed epitope structure is displayed as ribbon and the buried portion of the epitope is shown as a blue CA trace. H: Enolase epitope structure for basidiomycetes. The exposed epitope structure is displayed as ribbon and the buried portion of the epitope is shown as a blue CA trace. I: Structure and amino acid sequence of the divergent loop in yeast and basidiomycete epitopes (circled in C, D and E)