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Figure 1 | BMC Genomics

Figure 1

From: Design factors that influence PCR amplification success of cross-species primers among 1147 mammalian primer pairs

Figure 1

Examples of PCR results ascribed to subjective rankings used for cross-species amplifications shown in Table 3. Lane numbers and subjective rankings appear above and below the picture, respectively. Lane 1, 100 bp ladder (Invitrogen Corp.); lanes 2 and 3, "4" ranking (lane 2, MACF1 monkey; lane 3, MACF1 dog); lanes 4 and 5, "3" ranking (lane 4, GNB1 mouse; lane 5, ORC4L cat); lanes 6 and 7, "2" ranking (lane 6, DVL1 goat; lane 7, CDC7 hamster); lanes 8 and 9, "1" ranking (lane 8, DVL1 monkey; lane 9, DVL1 pig). Ranking numbers are also provided below each lane. A ranking of "0" signifies that no bands of any kind were seen (not shown).

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