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Table 2 Number of IS-mismatches vs. target amplification

From: Design factors that influence PCR amplification success of cross-species primers among 1147 mammalian primer pairs

 

Dog

Hamster

IS-Mismatches

Testeda

Productb

%

Tested

Productb

%

0

841

548

65.2

905

443

49.0

1

100

55

55.0

104

44

42.3

2

43

19

44.2

44

16

36.4

3

25

13

62.0

26

5

19.2

4 + 5

7

3

42.9

8

2

25.0

  1. aThese numbers do not include primer pairs for which the dog is an index species.
  2. bDog products were all confirmed by sequence analysis. Hamster products were inferred to be correct based upon a subjective ranking of 2, 3, and 4 (see Fig. 1 for ranking system).
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BMC Genomics

ISSN: 1471-2164

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