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Table 2 Number of IS-mismatches vs. target amplification

From: Design factors that influence PCR amplification success of cross-species primers among 1147 mammalian primer pairs

  Dog Hamster
IS-Mismatches Testeda Productb % Tested Productb %
0 841 548 65.2 905 443 49.0
1 100 55 55.0 104 44 42.3
2 43 19 44.2 44 16 36.4
3 25 13 62.0 26 5 19.2
4 + 5 7 3 42.9 8 2 25.0
  1. aThese numbers do not include primer pairs for which the dog is an index species.
  2. bDog products were all confirmed by sequence analysis. Hamster products were inferred to be correct based upon a subjective ranking of 2, 3, and 4 (see Fig. 1 for ranking system).