BMC Genomics

Table 4 PCR success vs. target GC content for zero IS-mismatch primer pairs

From: Design factors that influence PCR amplification success of cross-species primers among 1147 mammalian primer pairs

	dog			hamster
GC range	no. tested	successful PCR	%	no. tested	successful PCR^a	%
24–30	31	26	83.9	33	16	48.5
30–35	167	129	77.2	189	83	43.9
35–40	186	131	70.4	195	91	46.7
40–45	148	109	73.6	160	81	50.6
45–50	91	58	63.7	101	50	49.5
50–55	99	67	67.7	103	54	52.4
55–60	69	34	49.3	70	30	42.9
60–65	37	13	35.1	42	22	52.4
>65	10	2	20.0	12	4	33.3

^aSuccessful hamster amplifications are based upon subjective rankings of 2, 3, and 4 (see Fig. 1 for the ranking system).

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