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Table 4 PCR success vs. target GC content for zero IS-mismatch primer pairs

From: Design factors that influence PCR amplification success of cross-species primers among 1147 mammalian primer pairs

  dog hamster
GC range no. tested successful PCR % no. tested successful PCRa %
24–30 31 26 83.9 33 16 48.5
30–35 167 129 77.2 189 83 43.9
35–40 186 131 70.4 195 91 46.7
40–45 148 109 73.6 160 81 50.6
45–50 91 58 63.7 101 50 49.5
50–55 99 67 67.7 103 54 52.4
55–60 69 34 49.3 70 30 42.9
60–65 37 13 35.1 42 22 52.4
>65 10 2 20.0 12 4 33.3
  1. aSuccessful hamster amplifications are based upon subjective rankings of 2, 3, and 4 (see Fig. 1 for the ranking system).
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